Sunquest Code:MHGB  
Epic Code:LAB4187Epic Name:Methemoglobin
Methodology:Spectrophotometric analysis of hemolysate
CPT Code:83050
Turnaround Time:Performed and reported 24 hours/day.
Special Instructions:Sample is pH dependent. Methemoglobin may revert to hemoglobin on standing. Ideally, test is performed within 2 hours of collection; up to 10% decrease may occur after 4 hours, up to a 16% decrease after 8 hours.

Collection Instructions

Specimen:Blood (arterial, venous, capillary)
Optimal Volume:1 mL
Minimum\Peds Volume:0.5 mL (3 mL syringe); 0.2 mL (tuberculin syringe); 0.1 mL (capillary tube)
Container:Dry heparin syringe (Capillary (lithium heparin) gas tube, Green (lithium heparin, no gel), Green (lithium heparin) on ICE, Green (sodium heparin, no gel))
Collection Instructions:Sample should be free of air bubbles. If collecting in green (heparin) tube, do not remove stopper. Deliver to the laboratory immediately; if received within 20 minutes, ice is not required.
Causes for Rejection:Gross lipemia; presence of >1% sulfhemoglobin, >60 mg/L methylene blue or citrate.

Processing and Shipping

Specimen Processing:Do not store.
Shipping Instructions:Ship on wet ice to arrive within 8 hours, preferably 2 hours.
Test Performed at or Referral Lab Chemistry  (Fairview Range - FRMC, UMMC-East Bank, Fairview Ridges - FRH, Fairview Northland - FNMC, Fairview Southdale - FSH, UMMC/UMMCH-West Bank, UMMC NICU, M Health Clinic and Surgery Center)


Critical Range:Greater than 35%
Reference Range:0-3%; smokers may be slightly higher than nonsmokers.

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