HIV 1 Antibody Confirmation, Western Blot
| Sunquest Code: | HIABWB | ||
| Epic Code: | LAB864 | Epic Name: | HIV 1 Antibody Western Blot Confirm |
| Synonyms: | Human Immunodeficiency Virus Type 1 Antibody, Western Blot | ||
| Methodology: | Western blot | ||
| Turnaround Time: | Specimens are sent to reference laboratory Mon-Sat; results are reported within 3 days. | ||
| Special Instructions: | Order this assay only when a specimen is repeatedly reactive for HIV 1 or HIV 1-2 antibodies. | ||
Collection Instructions
| Specimen: | Blood |
| Optimal Volume: | 2 mL |
| Minimum\Peds Volume: | 1.2 mL |
| Container: | Red or gold (gel) (Blue Na Citrate, 3.2% - fill to fill line on tube, Green (lithium heparin, gel), Green (sodium heparin, no gel), Purple (EDTA), Red (no gel)) |
| Causes for Rejection: | Hemolyzed or lipemic specimens. |
Processing and Shipping
| Specimen Processing: | Separate serum from cells ASAP. Centrifuge and aliquot 1 mL, 0.5 mL minimum. Store in refrigerator. |
| Shipping Instructions: | Ship at refrigerated temperature. |
| Stability: | After separation from cells: Up to 1 week at room temperature is acceptable but not preferred; 1 week refrigerated; indefinitely frozen. |
| Test Performed at or Referral Lab | Lab Sendouts (ARUP) |
| Referral Lab number: | 20284 |
Interpretive
| Reference Range: | Negative |
| Use: | Assay intended for clinical diagnosis and should not be considered for blood donor screening or associated re-entry protocols, or for screening human cells, tissues and cellular and tissue based products. |
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