Prothrombin Fragment 1.2


Abbrev Code:PF   
Order Code:LAB3671Order Name:Prothrombin Fragment
Methodology:Enzyme-linked immunosorbent assay
CPT Codes: 85397 x1, 85397 x1
Turnaround Time:Turnaround time is 7 days, testing performed 1 day per week (Monday or Tuesday). With pre-notification, STATs are done within 1 hour after receipt in the testing laboratory.
Associated Links:

Specimen Collection and Processing for Coagulation Specimens


Collection Instructions

Specimen:Blood
Optimal Volume:2.7 mL (in 3 mL tube) - filled to fill line on the tube.
Minimum\Peds Volume:1.8 mL (in 2 mL tube) - filled to fill line on the tube.
Container:Blue Na Citrate, 3.2% - fill to fill line on tube
Collection Instructions:Deliver to the laboratory within 3 hours. See link to Specimen Collection and Processing for Coagulation Specimens. Aliquot into 2 tubes.
Causes for Rejection:Underfilled tubes, overfilled tubes, clotted specimens, not received or processed and frozen within 4 hours of collection or, specimen thawed during transit.


Processing and Shipping

Specimen Processing:
  • Process for Platelet Poor Plasma within 4 hours of collection.
  • Aliquot plasma, place clear tape around label, and freeze at -20C or on dry ice within 4 hours of collection.
  • If multiple tests are collected on 1 blue top, split each blue top into 2 aliquots. Minimum 0.5 mL per tube.
  • A maximum of 6 aliquots are needed per collection.
     
See link to Specimen Collection and Processing for Coagulation Specimens.
Shipping Instructions:
  • Ensure specimen is frozen before shipping.
  • Ship on dry ice, sample must remain frozen during shipping.
     
See link to Specimen Collection and Processing for Coagulation Specimens.
Test Performed at or Referral Lab UM Special Coag (K)  (UMMC East Bank)


Interpretive

Reference Range:Less than 0.35 nmol/L
Use:Determines if a fragment of Prothrombin called F1.2 is present. This fragment is produced when the patient's coagulation system is activated.


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