Phenobarbital Level
| Sunquest Code: | PHENO | ||
| Epic Code: | LAB30 | Epic Name: | Phenobarbital Level |
| Synonyms: | Also see Free Phenobarbital (FPHENO, LAB3482) | ||
| Methodology: | Turbidmetric rate
GH: Homogenous enzyme immunoassay SJO/SJN: Chemiluminescent microparticle immunoassay (CMIA) |
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| Turnaround Time: | Performed and reported 24 h/day. | ||
Collection Instructions
| Specimen: | Blood |
| Optimal Volume: | 2.5 mL |
| Minimum\Peds Volume: | 0.8 mL |
| Container: | Red (no gel), St Johns/St Joseph/Woodwinds-Green (lithium heparin, no gel) [Alternate Containers: Green (lithium heparin, no gel), Grand Itasca - Green (lithium heparin, no gel), St Johns/St Joseph/Woodwinds - Red (no gel)] |
| Collection Instructions: | Trough level is preferred; collect immediately before dose. It is advisable to monitor at the same time in the dosing schedule. |
| Causes for Rejection: | Collection in a gel tube. |
Processing and Shipping
| Specimen Processing: | Centrifuge and aliquot 1.25 mL, 0.3 mL minimum. Store in room temperature up to 48 hours. Alternatively, refrigerate. |
| Shipping Instructions: | Ship at room temperature to arrive within 48 hours. Alternatively, ship at refrigerated temperature. |
| Stability: | Stability: Room temperature - 48 hours; refrigerated - 1 month; frozen - 3 months. |
| Test Performed at or Referral Lab | Chemistry (Range, UMMC-West Bank, UMMC-East Bank, Southdale, Grand Itasca, St. Joseph's) |
Interpretive
| Critical Range: | Greater than 50 mg/L |
| Therapeutic Range: | 15-40 mg/L
Grand Itasca: 10-40 ug/mL |
| Use: | Monitor therapeutic drug level of phenobarbital; use with primidone levels to monitor therapeutic drug level of primidone; evaluate phenobarbital toxicity. Half-life is 50-120 h (adult); and 40-70 h (child). |
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