Blood Culture, Aerobic

Sunquest Code:BC  
Epic Code:LAB462Epic Name:Blood Culture
Synonyms:BCX; Blood CX; B CX
Methodology:BACTEC culture method (fluorescence)
Test Includes:Culture for aerobes, anaerobes (see below).
Turnaround Time:Performed 24 hours/day; preliminary reports are issued at 24 hours. Care provider is contacted when culture is positive; negative results are reported within 6 days.
Special Instructions:
Previous antimicrobial therapy may delay the growth of organisms but does not warrant additional cultures. Note if blood specimen is collected through a vascular access device to aid in interpretation of positive results.

Aerobic and anaerobic culture bottles are always drawn together from each collection site for each collection.
Associated Links:

Blood Collection for Cultures (Bacterial, Fungal, Viral), BACTEC Training

Collection Instructions

Optimal Volume:Adult: 8-10 mL, 3mL minimum, in BACTEC Plus Aerobic/F
Minimum\Peds Volume:Pediatric: 3 mL, 1 mL minimum in BACTEC Peds Plus/F
Container:Blood Culture Bottle(s)
Collection Instructions:
See Blood Collection for Cultures (Bacterial, Fungal, Viral) under Laboratory Procedures.

Collect both aerobic and anaerobic cultures from each site at each collection.

FUO, acute or subacute bacterial endocarditis: Obtain three blood cultures on the first day, at least 10 minutes apart. If negative after 24 hours, collect two or three more. If five to seven blood cultures are negative, consult the Microbiology Laboratory and Infectious Disease. Special culture techniques may be useful. Indicate if endocarditis is suspected on request form.  
Patient Preparation:
See Blood Collection for Cultures (Bacterial, Fungal, Viral) under Laboratory Procedures.  

Processing and Shipping

Specimen Processing:Store at room temperature.
Shipping Instructions:Ship at room temperature. Must arrive within 24 hours.
Test Performed at or Referral Lab Infectious Diseases Diagnostic Laboratory  (UMMC-East Bank, Grand Itasca GICH)


Critical Range:Any bacterial isolate should be considered significant and evaluated in relation to patient's clinical state.
Reference Range:No growth.
Use:Isolate and identify potentially pathogenic organisms causing bacteremia or endocarditis.

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