KOH Preparation


Abbrev Code:KOHSTN   
Order Code:LAB251Order Name:KOH Prep
Synonyms:Fungal Smear; Potassium Hydroxide Preparation; Fungus
Methodology:Direct examination after 20% KOH hydrolysis of protein debris and cells.
CPT Codes: 87210 x1
Test Includes:Direct examination of specimen for fungal elements.
Turnaround Time:Performed 24 hours/day, results are reported within 4 hours.
Special Instructions:The same specimen may be submitted for bacterial and fungal culture and KOH wet mount.


Collection Instructions

Specimen:Sputum, bronchial aspirate, tissue, pus, granules, genital sources or corneal scraping. Not performed on blood, bone marrow, CSF or urine; however, these sources are recommended for fungal culture.
Optimal Volume:Visible material
Container:Plastic leakproof container, sterile
Alternate Containers: CoPan ESwab with larger swab
Collection Instructions:Refer to directions for collection of Fungus Culture. ESwab may be used for collection for oral thrush diagnosis.
Causes for Rejection:Specimen collected with any swab other than ESwab.


Processing and Shipping

Specimen Processing:Store at room temperature.
Shipping Instructions:Ship at room temperature. Must arrive within 24 hours.
Test Performed at or Referral Lab Infectious Diseases Diagnostic Laboratory  (Clinics, CSC Maple Grove, Grand Itasca, UMMC East Bank)


Interpretive

Reference Range:No fungal elements seen.
Use:Determine presence of fungi in respiratory or genital sources. If encapsulated yeast are observed there is a high correlation with the presence of Cryptococcus neoformans, viable or non-viable forms.


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