Arterial Blood Gas With Oxyhemoglobin

Sunquest Code:BGAOB  
Epic Code:LAB4561Epic Name:Blood Gas Arterial and OXYHGB
Order Instructions:Use test code for arterial (BGAOB), venous (BGVOB), capillary (BGCOB), or type unknown (BGBOB). No reference ranges print with BGBOB. Order ELS (ECMO) samples as BGEV (VEN) or BGEA (ART).
Synonyms:ABG; Arterial Blood Gas; Oxygen Saturation; Oxyhemoglobin; pH; pCO2; pO2
Methodology:Specific electrodes (pH, pCO2, pO2); spectrophotometric analysis of hemolysate: %OxyHb = cOxyHb/ctHb; %O2Sat = cOxyHb/(ctHb -[cCOHb + cMetHb])
Test Includes:pH, pCO2, pO2, derived HCO3, oxyhemoglobin
Turnaround Time:Performed and reported 24 hours/day.
Special Instructions:UMMC and FNMC: Whole blood sodium, potassium, chloride, CO2, ionized calcium, lactate and glucose may be requested on this specimen. Provide FIO2 on request form. Whole blood potassium is stable at room temperature for 2 hours. Whole blood potassium is stable on ice/gel packs for 30 minutes.

Collection Instructions

Optimal Volume:1 mL
Minimum\Peds Volume:0.5 mL (3 mL syringe); 0.2 mL (tuberculin syringe)
Container:Dry heparin syringe
Collection Instructions:
Specimen should be free of air bubbles. Deliver to laboratory immediately; if received within 30 minutes, ice is not required.

Arterial Puncture: Use VENTED, dry heparin syringe.
  • Adjust syringe plunger to desired fill volume (0.5 mL)
  • Remove needle sheath.
  • Prepare puncture site and perform puncture. Syringe fills when artery is punctured, and "seals" when filled to pre-selected volume.
  • Withdraw needle, remove any bubbles present, and mix syringe thoroughly.

Note: If using vented syringe for collection from vein or vascular device, push plunger all the way in before starting procedure. Aspirate sample as with non-vented syringe by placing thumb over center hole of plunger.  

Processing and Shipping

Specimen Processing:Do not store.
Shipping Instructions:Ship on wet ice to arrive within 2 hours. UMMC, FNMC and FLMC only: Do not send in PTS.
Test Performed at or Referral Lab Chemistry  (University of Minnesota Health Maple Grove Clinics, Range, UMMC-East Bank, Lakes, Ridges, Northland, Southdale, UMMC/UMMCH-West Bank, UMMC NICU, Grand Itasca)


Critical Range:
pH Less than 7.20 Greater than 7.60
pCO2 Less than 20 mmHg Greater than 75 mmHg
pO2, arterial Less than 40 mmHg  
Derived HCO3 Less than 11 mmol/L Greater than 45 mmol/L
Reference Range:
Arterial pH pCO2 pO2 dHCO3 Base Excess
    mm Hg mm Hg mmol/L  
0-1 y 7.35-7.45 26-40 80-105 16-24 -9.0 to1.08
2 y & older 7.35-7.45 35-45 80-105 21-28 -9.0 to 1.08
Oxyhemoglobin (arterial):  92-100%

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