Gene Rearrangement, T-Cell Receptor, PCR


Abbrev Code:GNRTMD   
Order Code:LAB4745Order Name:T Cell Receptor Gene Rearrangement PCR
Synonyms:T-Cell Receptor Rearrangement; Gene-T; Molecular
Methodology:Amplification of DNA and detection by capillary electrophoresis.
CPT Codes: 81342 x1, G0452 x1
Test Includes:T-cell receptor gamma chain rearrangement by PCR.
Turnaround Time:Results are reported within 10-14 days.


Collection Instructions

Specimen:Blood
Optimal Volume:10 mL blood; 5 mL bone marrow; 5 mm x 5 mm x 5 mm fresh tissue, embedded tissue (sufficient for 60-100 five micron sections)
Minimum\Peds Volume:5 mL blood; 2 mL bone marrow; 2 mm x 3 mm x 3 mm fresh tissue
Container:Yellow (ACD, Solution A) tube available from laboratory
Alternate Containers: Purple (EDTA)
Collection Instructions:
 
  • Peripheral Blood: Collect 10 mL peripheral blood in yellow (ACD) tube; alternate container: purple (EDTA) tube.
  • Bone marrow aspirate: Aspirate ACD (or EDTA) solution into syringe from open ACD (or EDTA) tube; collect 5 mL bone marrow into syringe. Deliver in syringe.
  • Fresh tissue: Collect in sterile container; deliver to the laboratory within 1 hour, or freeze specimen (liquid nitrogen or dry ice) and transport to the laboratory frozen (-70 C) or place in RPMI and transport same day at 4 C.
  • Paraffin embedded tissue: Indicate fixative used.
 
Causes for Rejection:
Clotted or frozen blood or bone marrow, necrotic tissue, incorrect anticoagulant, specimen more than 5 days old, clinically not indicated.
Samples cannot be shared with hematology testing, contamination will occur on automated hematology analyzers.    


Processing and Shipping

Specimen Processing:Store at room temperature. Do not freeze.
Shipping Instructions:Ship blood, bone marrow, and fixed tissue at room temperature to arrive within 24 h. Ship fresh tissue at RT to arrive within 1 h, in RPMI at 4 C to arrive same day, on dry ice to arrive within 24 h.
Stability:After hours and weekends, store blood and bone marrow room temp. Tissue must arrive within 1 hour of collection, be frozen at -70C, or put in RPMI.
Test Performed at or Referral Lab UM Molecular Diagnostics (M)  (UMMC East Bank)


Interpretive

Reference Range:Results are reported as negative (germline or no rearrangement noted) or positive (clonal rearrangement indicating clonal cell populations present).
Use:Evaluation for clonal rearrangements of the T-cell receptor genes and adjunct in diagnosing lymphomas and leukemias of T-cell lineage.


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