Gene Rearrangement, T-Cell Receptor, PCR


Sunquest Code:GNRTMDCoPath Code:GNRTPCR
Epic Code:LAB4745Epic Name:T Cell Receptor Gene Rearrangement PCR
Synonyms:T-Cell Receptor Rearrangement; Gene-T; Molecular
Methodology:Amplification of DNA and detection by capillary electrophoresis.
Test Includes:T-cell receptor gamma chain rearrangement by PCR.
Turnaround Time:Results are reported within 10-14 days.


Collection Instructions

Specimen:Blood
Optimal Volume:10 mL blood; 5 mL bone marrow; 5 mm x 5 mm x 5 mm fresh tissue, embedded tissue (sufficient for 60-100 five micron sections)
Minimum\Peds Volume:5 mL blood; 2 mL bone marrow; 2 mm x 3 mm x 3 mm fresh tissue
Container:Yellow (ACD, Solution A) tube available from laboratory (Purple (EDTA))
Collection Instructions:
 
  • Peripheral Blood: Collect 10 mL peripheral blood in yellow (ACD) tube; alternate container: purple (EDTA) tube.
  • Bone marrow aspirate: Aspirate ACD (or EDTA) solution into syringe from open ACD (or EDTA) tube; collect 5 mL bone marrow into syringe. Deliver in syringe.
  • Fresh tissue: Collect in sterile container; deliver to the laboratory within 1 hour, or freeze specimen (liquid nitrogen or dry ice) and transport to the laboratory frozen (-70 C) or place in RPMI and transport same day at 4 C.
  • Paraffin embedded tissue: Indicate fixative used.
 
Causes for Rejection:
Clotted or frozen blood or bone marrow, necrotic tissue, incorrect anticoagulant, specimen more than 5 days old, clinically not indicated.
Shared blood or bone marrow samples will not be accepted unless sent to Molecular first. Contamination will occur on automated hematology analyzers.    


Processing and Shipping

Specimen Processing:Store at room temperature. Do not freeze.
Shipping Instructions:Ship blood, bone marrow, and fixed tissue at room temperature to arrive within 24 h. Ship fresh tissue at RT to arrive within 1 h, in RPMI at 4 C to arrive same day, on dry ice to arrive within 24 h.
Stability:After hours and weekends, store blood and bone marrow room temp. Tissue must arrive within 1 hour of collection, be frozen at -70C, or put in RPMI.
Test Performed at or Referral Lab Molecular Diagnostics  (UMMC-East Bank)


Interpretive

Reference Range:Results are reported as negative (germline or no rearrangement noted) or positive (clonal rearrangement indicating clonal cell populations present).
Use:Evaluation for clonal rearrangements of the T-cell receptor genes and adjunct in diagnosing lymphomas and leukemias of T-cell lineage.


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