Gene Rearrangement, T-Cell Receptor, PCR
Abbrev Code: | GNRTMD | ||
Order Code: | LAB4745 | Order Name: | T Cell Receptor Gene Rearrangement PCR |
Synonyms: | T-Cell Receptor Rearrangement; Gene-T; Molecular | ||
Methodology: | Amplification of DNA and detection by capillary electrophoresis. | ||
CPT Codes: | 81342 x1, G0452 x1 | ||
Test Includes: | T-cell receptor gamma chain rearrangement by PCR. | ||
Turnaround Time: | Results are reported within 10-14 days. |
Collection Instructions
Specimen: | Blood |
Optimal Volume: | 10 mL blood; 5 mL bone marrow; 5 mm x 5 mm x 5 mm fresh tissue, embedded tissue (sufficient for 60-100 five micron sections) |
Minimum\Peds Volume: | 5 mL blood; 2 mL bone marrow; 2 mm x 3 mm x 3 mm fresh tissue |
Container: | Yellow (ACD, Solution A) tube available from laboratory Alternate Containers: Purple (EDTA) |
Collection Instructions: |
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Causes for Rejection: |
Clotted or frozen blood or bone marrow, necrotic tissue, incorrect anticoagulant, specimen more than 5 days old, clinically not indicated.
Samples cannot be shared with hematology testing, contamination will occur on automated hematology analyzers. |
Processing and Shipping
Specimen Processing: | Store at room temperature. Do not freeze. |
Shipping Instructions: | Ship blood, bone marrow, and fixed tissue at room temperature to arrive within 24 h. Ship fresh tissue at RT to arrive within 1 h, in RPMI at 4 C to arrive same day, on dry ice to arrive within 24 h. |
Stability: | After hours and weekends, store blood and bone marrow room temp. Tissue must arrive within 1 hour of collection, be frozen at -70C, or put in RPMI. |
Test Performed at or Referral Lab | UM Molecular Diagnostics (M) (UMMC East Bank) |
Interpretive
Reference Range: | Results are reported as negative (germline or no rearrangement noted) or positive (clonal rearrangement indicating clonal cell populations present). |
Use: | Evaluation for clonal rearrangements of the T-cell receptor genes and adjunct in diagnosing lymphomas and leukemias of T-cell lineage. |
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