PRA Single Antigen, IgG
| Abbrev Code: | PRAIGG | ||
| Order Code: | LAB6447 | Order Name: | PRA Single Antigen, IgG Antibody |
| Order Instructions: | If this test is being added on, comment ADD-ON in the Comment field when placing a normal order in Epic. | ||
| Synonyms: | WBC Antibody; White Cell Antibody; HLA Class I/II Antibody; PRA (Panel Reactive Antibody); HLA Antibody; IMM3; SOT PRA | ||
| Methodology: | Luminex solid phase assay | ||
| CPT Codes: | 86832 x1, 86832 x1, 86833 x1, 86833 x1 | ||
| Test Includes: | Patient's serum tested against purified HLA antigens to determine the presence of HLA Class I and/or HLA Class II antibody. Reflex testing, as determined by the laboratory, to determine the percent reactivity and/or specificity of the HLA antibodies present. | ||
| Turnaround Time: | Results are reported within 7 days. | ||
Collection Instructions
| Specimen: | Blood |
| Optimal Volume: | 14 mL |
| Minimum\Peds Volume: | 3 mL |
| Container: | Red (no gel) Alternate Containers: Red (gel) |
| Collection Instructions: | Collect specimen prior to dialysis. Do not collect specimen if patient has received IVIG, OKT3 or Campath recently; these drugs may case false positive results. Inform lab if patient has received anti-thymoglobulin. Lab must remove serum prior to testing. |
| Causes for Rejection: | Gross hemolysis; immunosuppression drug interference |
| Contraindications: | Serum will not clot if collected during or immediately after dialysis. |
Processing and Shipping
| Specimen Processing: | Do not process (preferred). If processed, remove serum. Store at room temperature. |
| Shipping Instructions: | Ship at room temperature; protect from temperature extremes. |
| Stability: | Store at room temperature (preferred), refrigerated or frozen. |
| Test Performed at or Referral Lab | Immunology (UMMC East Bank) |
Interpretive
| Use: | Detection of HLA Class I and/or Class II antibodies in patient serum. High levels of HLA antibody may result in difficulty in finding a compatible organ or marrow donor. |
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