PRA Single Antigen, IgG

Sunquest Code:PRAIGG  
Epic Code:LAB6447Epic Name:PRA Single Antigen, IgG Antibody
Order Instructions:If this test is being added on, comment ADD-ON in the Comment field when placing a normal order in Epic. Immunology will receive the printout directly. All other lab areas should discard the Immunology ADD-ON printout.
Synonyms:WBC Antibody; White Cell Antibody; HLA Class I/II Antibody; PRA (Panel Reactive Antibody); HLA Antibody; IMM3; SOT PRA
Methodology:Luminex solid phase assay
Test Includes:Patient's serum tested against purified HLA antigens to determine the presence of HLA Class I and/or HLA Class II antibody. Reflex testing, as determined by the laboratory, to determine the percent reactivity and/or specificity of the HLA antibodies present.
Turnaround Time:Results are reported within 7 days.

Collection Instructions

Optimal Volume:14 mL
Minimum\Peds Volume:3 mL
Container:Red (no gel)
[Alternate Containers: Red (gel)]
Collection Instructions:Collect specimen prior to dialysis. Do not collect specimen if patient has received IVIG, OKT3 or Campath recently; these drugs may case false positive results. Inform lab if patient has received anti-thymoglobulin. Lab must remove serum prior to testing.
Causes for Rejection:Gross hemolysis; immunosuppression drug interference
Contraindications:Serum will not clot if collected during or immediately after dialysis.

Processing and Shipping

Specimen Processing:Do not process. Store at room temperature.
Shipping Instructions:Ship at room temperature; protect from temperature extremes.
Test Performed at or Referral Lab Immunology  (UMMC-East Bank)


Use:Detection of HLA Class I and/or Class II antibodies in patient serum. High levels of HLA antibody may result in difficulty in finding a compatible organ or marrow donor.

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