Entamoeba histolytica (amebiasis), Antibody, IgG

Abbrev Code:EHIST   
Order Code:LAB3448Order Name:E histolytica Antibody
Synonyms:Amebiasis Antibody; Amebiasis histolytica Antibody; E. histolytica Antibody
Methodology:Enzyme-linked immunosorbent assay
CPT Codes: 86753 x1
Turnaround Time:Specimens are sent to reference laboratory Mon-Sat; results are reported in 2-6 days.
Special Instructions:Parallel testing is preferred. Label samples as acute or convalescent. Convalescent specimens must be received within 30 days from receipt of the acute specimens.

Collection Instructions

Optimal Volume:2 mL
Minimum\Peds Volume:0.4 mL
Container:Red or gold (gel)
Alternate Containers: Red (no gel)
Causes for Rejection:Hemolyzed, heat-inactivated, lipemic or contaminated samples.

Processing and Shipping

Specimen Processing:Separate serum from cells ASAP. Centrifuge and aliquot 1 mL, 0.1 mL minimum.
Shipping Instructions:Ship on dry ice.
Stability:48 hours at room temperature; 2 weeks refrigerated; 1 year frozen.
Test Performed at or Referral Lab Lab Sendouts  (ARUP)
Referral Lab number:50070 (AMB)


Reference Range:
8 U or less Negative: No significant level of detectable
E. histolytica IgG antibody.
9-11 U Equivocal: Repeat testing in 10-14 days may be helpful.
12 U or greater Positive: IgG antibody to E. histolytica detected, which may indicate a current or recent infection.
  Seroconversion between acute and convalescent sera is considered strong evidence of recent infection. The best evidence for infection is a significant change on two appropriately timed specimens where both tests are done in the same laboratory at the same time. Note: In the case of extra-intestinal complications, a positive antibody can indicate amebiasis, even though stool findings are negative  

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