FISH (Fluorescent in Situ Hybridization) Interphase


Abbrev Code:FISHCG   
Order Code:LAB4736  
Synonyms:LAB1401; FISH Interphase: ALL, AML, CLL, CML, MDS, Lymphoma; Neoplastic, Cancer
Methodology:FISH Interphase
CPT Codes: 88275 x1, 88271 x1, 88291 x1, 88237 x1, 88230 x1, 88387 x1, 88233 x1, 88333 x1
Turnaround Time:Results reported within 21 days.
Special Instructions:Consultation must occur with Cytogenetics Lab (612-273-3171) prior to order. Charges will vary with type of probe required, as determined by Cytogenetics Lab. Each probe ordered will accrue a probe and analysis charge.
Test request form requires:
  • Date and time of specimen collection
  • Specimen type
  • Patient information: date of birth, sex and medical record number
  • Ordering physician or genetic counselor, and contact numbers
  • Reason for referral
  • Clinical status
  • Information regarding medications or transfusions
  • Previous or pending genetic testing results

A signed informed consent in the patient's medical record is required. The consent should not be sent to the laboratory. A link to the Genetic Testing Consent Form is provided as a convenience for the providers and genetic counselors. See Associated Links.
Associated Links:

Genetic Testing Consent Form


Collection Instructions

Specimen:Bone Marrow, Blood, Cord Blood, Tissue Parrafin, Embeded Tissue Slidesv(FFPE), Products of Conception, CSF/Cytospin Slides
Optimal Volume:
  • Whole Blood or Cord Blood: 10 mL
  • Bone Marrow: 5 mL bone marrow aspirate
  • Tissue: 1 cm3
  • Paraffin Embedded Slides: For each probe ordered, two unstained and one H&E slide on positively charged slides, 4-6 microns thick. Slides should contain two patient identifiers. Must have patient name and one of the following identifiers: date of birth, medical record number, pathology number.
  • Products of Conception: 35-55 mg of Villi
Minimum\Peds Volume:
  • Whole Blood or Cord Blood: 5 mL
  • Bone Marrow: 3 mL bone marrow aspirate
  • Tissue: 5 mm3
  • Paraffin Embedded Slides: Tissue should be cut onto positively charged slides, 4-6 microns thick. Slides should contain two patient identifiers. Must have patient name and one of the following identifiers: date of birth, medical record number, pathology number.
  • Products of Conception: Collect in plastic leakproof sterile container containing RPMI media. Sterile saline may be used if RPMI is not available.
Container:Green (sodium heparin, no gel)
Alternate Containers: Plastic leakproof container, sterile, Container, Tissue, sterile, Slides, Dry heparin syringe
Collection Instructions:
  • Bone marrow aspirate: Dry heparin syringe. 
  • Whole Blood or Cord Blood: Green (sodium heparin, no gel) tube.
  • Products of Conception: Collect in plastic leakproof sterile container containing RPMI  media. Sterile saline may be used if RPMI is not available.
  • Fresh Tissue: Collect in tissue culture media.
  • Paraffin Embedded Slides: Cut onto positively charged slides.
Causes for Rejection:Clotted or frozen specimen. Incorrect specimen collection tube (anticoagulant).
Paraffin Embedded Slide: Not on a positively charged slide. No material on the unstained slide. Tissue is cut too thick/thin. Decalcified tissue.


Processing and Shipping

Specimen Processing:Do not process. Store at room temperature. Do not refrigerate or freeze.
Specimens must be received in the Cytogenetics Laboratory Mon-Fri by 5:30 pm. Weekends and holidays by 4:30 pm. Specimens received after these cutoffs will be processed the following day.
Shipping Instructions:Ship at room temperature. For optimal testing results the specimen must arrive within 24 hours.
Stability:
 

 

Test Performed at or Referral Lab Cytogenetics  (UMMC East Bank)


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