FISH (Fluorescent in Situ Hybridization) Interphase


Sunquest Code:FISHCGCoPath Code:FISHINTER
Epic Code:LAB4736Epic Name:FISH Interphase
Sunquest Code (non-Epic sites):FISHCG  
Synonyms:FISH Interphase: ALL, AML, CLL, CML, MDS, Lymphoma
Methodology:FISH Interphase
Turnaround Time:Results reported within 21 days.
Special Instructions:Consultation must occur with Cytogenetics Lab (612-273-3171) prior to order. Charges will vary with type of probe required, as determined by Cytogenetics Lab. Each probe ordered will accrue a probe and analysis charge. Test request form requires:
  • Date and time of specimen collection
  • Specimen type
  • Patient information: date of birth, sex and medical record number
  • Ordering physician or genetic counselor, and contact numbers
  • Reason for referral
  • Clinical status
  • Information regarding medications or transfusions
  • Previous or pending genetic testing results
Associated Links:

Genetic Testing Consent Form


Collection Instructions

Specimen:Bone Marrow, Blood, Tissue Parrafin, Embeded Slides or Products of Conception
Optimal Volume:
  • Blood: 10 mL
  • Bone Marrow: 5 mL bone marrow aspirate
  • Tissue: 1 cm3
  • Paraffin Embedded Slides: For each probe ordered, two unstained and one H&E slide on positively charged slides, 4-6 microns thick
  • Products of Conception: 35-55 mg of Villi
Minimum\Peds Volume:
  • Blood: 5 mL
  • Bone Marrow: 3 mL bone marrow aspirate
  • Tissue: 5 mm3
  • Paraffin Embedded Slides: Tissue should be cut onto positively charged slides, 4-6 microns thick. Slides should contain two patient identifiers. Must have patient name and one of the following identifiers: date of birth, medical record number, pathology number.
  • Products of Conception: Collect in plastic leakproof sterile container containing RPMI media. Sterile saline may be used if RPMI is not available.
Container:Green (sodium heparin, no gel) (Plastic leakproof container, sterile, Container, Tissue, sterile, Slides, Dry heparin syringe)
Collection Instructions:Bone marrow aspirate: Dry heparin syringe.  Blood: Green (sodium heparin, no gel) tube.
Causes for Rejection:Clotted or frozen specimen. Incorrect specimen collection tube (anticoagulant).
Paraffin Embedded Slide: Not on a positively charged slide. No material on the unstained slide. Tissue is cut too thick/thin. Decalcified tissue.


Processing and Shipping

Specimen Processing:Do not process. Store at room temperature. Do not refrigerate or freeze.
Specimens must be received in the Cytogenetics Laboratory Mon-Fri by 5:30 pm. Weekends and holidays by 4:30 pm. Specimens received after these cutoffs will be processed the following day.
Shipping Instructions:Ship at room temperature. For optimal testing results the specimen must arrive within 24 hours.
Stability:
 

 

Test Performed at or Referral Lab Cytogenetics  (UMMC-East Bank)


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