Hepatitis B DNA Quantitative; Real-Time PCR
| Sunquest Code: | HBVPCR | ||
| Epic Code: | LAB6463 | Epic Name: | Hepatitis B DNA Quantitative |
| Synonyms: | HBV Viral Load; HBQNT; HEPB; Hep B | ||
| Methodology: | Real-time polymerase chain reaction (PCR) | ||
| Turnaround Time: | Testing is performed twice weekly; results are reported within 4 days. | ||
Collection Instructions
| Specimen: | Blood |
| Optimal Volume: | 4 mL |
| Minimum\Peds Volume: | 2.2 mL |
| Container: | 10 mL Purple EDTA tube [Alternate Containers: Red or gold (gel)] |
| Causes for Rejection: | Heparinized samples, frozen whole blood, specimen types other than EDTA plasma or red or gold (gel) serum are not acceptable for testing. |
Processing and Shipping
| Specimen Processing: | Within 24 hours of collection, centrifuge and aliquot 2 mL, 1 mL minimum. Store refrigerated or frozen. |
| Shipping Instructions: | Ship at refrigerated or frozen temperature. |
| Stability: | Stability: Plasma or serum samples are stable at refrigerated temperature (2-8 degrees C) up to 7 days or frozen (-20 to -80 digress C) up to 40 days. |
| Test Performed at or Referral Lab | Infectious Diseases Diagnostic Laboratory (UMMC-East Bank) |
Interpretive
| Reference Range: |
Not detected. This assay has a result range of 20 to 170,000,000 IU/mL (1.3 to 8.23 log IU/mL).
A 'Not Detected' result indicates that the assay was unable to detect HBV DNA within the specimen.
A '<20 IU/mL (<1.3 log IU/mL)' result indicates that HBV DNA is detected, but the level present is less than the lower quantification limit of the assay.
A '>170,000,000 IU/mL (>8.23 log IU/mL)' result indicates that HBV DNA is detected, but the level present is above the upper quantification limit of the assay.
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| Use: |
This is a nucleic acid amplification test for the quantification of hepatitis B virus DNA in serum or plasma. Uses include: · Diagnose some cases of early acute HBV infection (before the appearance of HBsAg). · Distinguish active from inactive HBV infection. · Monitor response to anti-HBV therapy. · Quantification of HBV DNA in plasma/serum of patients with chronic HBV infection.
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