Hepatitis B DNA Quantitative; Real-Time PCR

Abbrev Code:HBVPCR   
Order Code:LAB6463Order Name:Hepatitis B DNA Quantitative
Synonyms:HBV Viral Load; HBQNT; HEPB; Hep B
Methodology:Real-time polymerase chain reaction (PCR)
CPT Codes: 87517 x1
Turnaround Time:Testing is performed twice weekly; results are reported within 4 days.

Collection Instructions

Optimal Volume:4 mL
Minimum\Peds Volume:2.2 mL
Container:10 mL Purple EDTA tube
Alternate Containers: Red or gold (gel)
Causes for Rejection:Heparinized samples, frozen whole blood, specimen types other than EDTA plasma or red or gold (gel) serum are not acceptable for testing.

Processing and Shipping

Specimen Processing:Within 24 hours of collection, centrifuge and aliquot 2 mL, 1 mL minimum. Store refrigerated or frozen.
Shipping Instructions:Ship at refrigerated or frozen temperature.
Stability:Stability: Plasma or serum samples are stable at refrigerated temperature (2-8 degrees C) up to 7 days or frozen (-20 to -80 digress C) up to 40 days.
Test Performed at or Referral Lab Infectious Diseases Diagnostic Laboratory  (UMMC-East Bank)


Reference Range:

Not detected. This assay has a result range of 20 to 170,000,000 IU/mL (1.3 to 8.23 log IU/mL).


A 'Not Detected' result indicates that the assay was unable to detect HBV DNA within the specimen.


A '<20 IU/mL (<1.3 log IU/mL)' result indicates that HBV DNA is detected, but the level present is less than the lower quantification limit of the assay.


A '>170,000,000 IU/mL (>8.23 log IU/mL)' result indicates that HBV DNA is detected, but the level present is above the upper quantification limit of the assay.



This is a nucleic acid amplification test for the quantification of hepatitis B virus DNA in serum or plasma. Uses include:

·          Diagnose some cases of early acute HBV infection (before the appearance of HBsAg).

·          Distinguish active from inactive HBV infection.

·          Monitor response to anti-HBV therapy.

·          Quantification of HBV DNA in plasma/serum of patients with chronic HBV infection.



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