Lymphocyte Antigen Proliferation


Sunquest Code:LYMAGP  
Epic Code:LAB6030Epic Name:Lymphocyte Antigen Proliferation
Order Instructions:When ordered with Lymphocyte Mitogens Proliferation, cancel LYMAGP and LYMMP and order LAM to combine.
Synonyms:Lymphocyte Blastogenesis, Antigens
Methodology:Cell culture
Turnaround Time:Specimens are sent to the reference laboratory Mon-Thur; results are reported in 9-10 days.
Special Instructions:Send sample in a STAT bag to East Bank Core Lab/Sendouts:

Time Sensitive Handling Required:
Specimen must be at the East Bank Sendouts lab no later than 1600. Order On-Time Delivery courier to deliver directly to East Bank Sendouts lab. Call Sendouts at 612-273-3711 to notify.
CSC and West Only: Send in red STAT bag by HealthEx courier. Call Sendouts at 612-273-3711 to notify.
Compliance:

This test was developed and its performance characteristics determined by ARUP Laboratories. It has not been cleared or approved by the U.S. Food and Drug Administration. This test was performed in a CLIA certified laboratory and is intended for clinical purposes.



Collection Instructions

Specimen:Blood
Optimal Volume:10 mL and a 10 mL normal control from an unrelated donor
Minimum\Peds Volume:7 mL (adult) or 3 mL (pediatric) and a 7 mL normal control from an unrelated donor
Container:Green (sodium heparin, no gel)
[Alternate Containers: Yellow (ACD, Solution A) tube available from laboratory]
Collection Instructions:Sample and sample must be collected Mon-Thur by noon. Specimen and control must be in the original tubes and arrive at the reference laboratory within 48 hours of collection time. Testing is performed Tue-Fri only.
Causes for Rejection:Refrigerated or frozen samples, or samples in transport longer than 48 hours. Samples collected in yellow (ACD Solution B)


Processing and Shipping

Specimen Processing:Whole blood. Do not process. Send sample and control at room temperature.
Shipping Instructions:Ship at room temperature. Must arrive within 48 hours. Ship to arrive Mon-Thur. Live lymphocytes required. Critical ambient; do not refrigerate or freeze.
Test Performed at or Referral Lab Lab Sendouts  (ARUP)
Referral Lab number:96055


Interpretive

Reference Range:By report.
Use: Candida and tetanus antigens are tested independently in lymphocyte culture. Lymphocyte proliferation in response to these antigens is determined by 3H-thymidine incorporation. Results are reported as the counts per minute (CPM) antigen stimulated versus a control culture and a stimulation index (SI) which represents the ratio of mean CPM of the stimulated lymphocytes to the mean CPM of the unstimulated control.


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