Lactic Acid, Whole Blood


Abbrev Code:LACTW   
Order Code:LAB730Order Name:Lactic Acid Whole Blood
Synonyms:Lactic Acid; Lactate for Lactate for Sepsis Protocol
Methodology:Lactate electrode
St. Johns/St Joseph/ Woodwinds: Lactic acid to pyruvate
CPT Codes: 83605 x1
Turnaround Time:Performed and reported 24 hours/day.


Collection Instructions

Specimen:Blood
Optimal Volume:1 mL
Minimum\Peds Volume:0.2 mL
Container:Lakes/Northland-Green (Lithium heparin, no gel) on ICE, St Johns/St Joseph/Woodwinds-Green (lithium heparin, no gel), Dry heparin syringe
Alternate Containers: Capillary (lithium heparin) gas tube, Green (lithium heparin) on ICE
Collection Instructions:Collect without the use of a tourniquet or within 3 minutes of applying the tourniquet, but before releasing the tourniquet. Do not allow patient to clench/unclench hand; this builds up lactic acid.

Heparinized plasma is accepetable, however, the following precautions must be rigidly adhered to in order to retard glycolysis: Collect on ice and transport immediately. Centrifuge within 15 minutes of collection to avoid metabolism of glucose to lactate. Separate the plasma immediately and store on ice until analysis.
Causes for Rejection:Moderate hemolysis
Contraindications:Lack of acidosis is not a contraindication for this test.


Processing and Shipping

Specimen Processing:Analyze whole blood as soon as possible - within 15 minutes at room temp, 60 minutes on ice.  If transport is required, centrifuge (ideally within 15 min.) at refrigerated temperature. Aliquot 0.2 mL, 0.1 mL minimum. Store on ice up to 24 hours; otherwise freeze immediately.
Shipping Instructions:Ship on dry ice.
Stability:Refer to Collection Instructions and Specimen Processing for stability.
Test Performed at or Referral Lab Chemistry  (Range, UMMC East, Lakes, Ridges, Northland, Southdale, UMMC/UMMCH-West Bank, UMMC West NICU, CSC Mpls, Grand Itasca, St. Joseph's, St. John's, Woodwinds)


Interpretive

Critical Range:Whole Blood Testing Critical Values and Reference Ranges
Reference Range:Whole Blood Testing Critical Values and Reference Ranges
Use:Identify lactic acidosis which characteristically presents as an increased anion gap metabolic acidosis.


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