Vitamin B1, Plasma - NOTE: Vitamin B1, Whole Blood is the preferred test

Abbrev Code:VITB1   
Order Code:LAB3874Order Name:Vitamin B1 Plasma
Order Instructions:Order this test ONLY if the physician order specifies a PLASMA Vitamin B1.
Methodology:High performance liquid chromatography
CPT Codes: 84425 x1
Turnaround Time:Specimens are sent to reference laboratory Mon-Sat; results are reported in 2-4 days.
Special Instructions:Whole blood is the preferred specimen for thiamine assessment. Approximately 80% of thiamine present in whole blood is found in red blood cells. Refer to Vitamin B1 (Thiamine), Whole Blood. This assay does not distinguish thiamine from its phosphate esters.

For tests developed and validated by ARUP (previously referred to as Compliance Statement B, C or D). This test was developed and its performance characteristics determined by ARUP Laboratories. It has not been cleared or approved by the U.S. Food and Drug Administration. This test was performed in a CLIA certified laboratory and is intended for clinical purposes.

Collection Instructions

Optimal Volume:2 mL
Minimum\Peds Volume:0.6 mL
Container:Purple (EDTA)
Alternate Containers: Green (sodium heparin, no gel)
Causes for Rejection:Non-frozen samples; hemolyzed sample. Samples other than heparin or EDTA plasma

Processing and Shipping

Specimen Processing:Centrifuge and aliquot within 1 hour of collection, 1 mL, 0.2 mL minimum. Each vitamin level requested requires a separate aliquot tube. Store frozen.
Shipping Instructions:Ship on dry ice.
Stability:After separation from cells: 1 week refrigerated; 6 months frozen.
Test Performed at or Referral Lab Lab Sendouts  (ARUP)
Referral Lab number:80389


Reference Range:4-15 nmol/L. Thiamine (vitamin B1) is reported. However, thiamine diphosphate (TDP), the biologically active form of thiamine, is not found in measurable concentration in plasma, and is best determined in whole blood specimens. Plasma thiamine concentration reflects recent intake rather than body stores.
Use:Rarely indicated. Whole blood is the specimen of choice for thiamine assessment.

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