BCR-ABL1: Molecular Detection of Minor Breakpoint Region, p190, Quantitative
| Sunquest Code: | ALLQT | CoPath Code: | BCRMINPCR |
| Epic Code: | LAB6579 | Epic Name: | BCRminqt BCR-ABL minor breakpoint Quant ALL |
| Sunquest Code (non-Epic sites): | MD | ||
| Synonyms: | Breakpoint Cluster Region (BCR) Analysis; Philadelphia Chromosome | ||
| Methodology: | Reverse transcription of mRNA (RT-PCR), amplification of cDNA for quantitative detection of ALL (minor) type breakpoint; real time PCR. | ||
| Turnaround Time: | Performed once per week; results are reported within 10 days. | ||
Collection Instructions
| Specimen: | Blood or bone marrow. RNA. |
| Optimal Volume: | 10 mL blood; 3 mL bone marrow |
| Minimum\Peds Volume: | 5 mL blood; 1 mL bone marrow; 25 uL RNA at a concentration of 20 ng/uL. |
| Container: | Yellow (ACD, Solution A) tube available from laboratory (Purple (EDTA)) |
| Collection Instructions: |
Note: The use of an anticoagulant other than ACD (or EDTA) may interfere with the assay. |
| Causes for Rejection: | Blood or bone marrow specimen received more than 72 hours from the date of collection; incorrect volume or tube type; clotted or frozen specimens; RNA not received on dry ice. RNA extracted at non-CLIA certified (or equivalent) lab.
Shared samples will not be accepted unless sent to Molecular first. Contamination will occur on automated hematology analyzers. |
Processing and Shipping
| Specimen Processing: | Store blood or bone marrow in refrigerator Do not freeze blood or bone marrow. Store RNA on dry ice. |
| Shipping Instructions: | Ship blood or bone marrow at refrigerated temperature to arrive within 24 hour of collection, Mon-Fri only. See Cause for Rejection. Ship RNA on dry ice. If shipping on Thursday or Friday, contact lab at 612-273-8445. |
| Test Performed at or Referral Lab | Molecular Diagnostics (UMMC-East Bank) |
Interpretive
| Reference Range: | By report. |
| Use: | Monitor the tumor load, status of remission, the minimal residual disease and chronological expression of level of the fusion transcripts for CML and AML patients. |
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