Borrelia burgdorferi Antibodies, IgG and IgM by Immunoblot (CSF)


Abbrev Code:CLYMGM   
Order Code:LAB3391Order Name:Lyme IGG and IGM CSF by Immunoblot
Synonyms:Lyme Antibody Detection; Lyme Disease, CSF
Methodology:Immunoblot
CPT Codes: 86617 x1, 86617 x1
Turnaround Time:Specimens are sent to reference laboratory Sun-Sat; results reported within 1-3 days.
Special Instructions:Parallel testing is preferred. Label samples as acute or convalescent.
Compliance:

For tests developed and validated by ARUP (previously referred to as Compliance Statement B, C or D). This test was developed and its performance characteristics determined by ARUP Laboratories. It has not been cleared or approved by the U.S. Food and Drug Administration. This test was performed in a CLIA certified laboratory and is intended for clinical purposes.


Collection Instructions

Specimen:CSF
Optimal Volume:3 mL
Minimum\Peds Volume:2 mL
Container:Sterile tube
Causes for Rejection:Hemolyzed, contaminated, xanthochromic or heat-inactivated specimens.


Processing and Shipping

Specimen Processing:Aliquot 3 mL, 2 mL minimum in an ARUP standard transport tube. Store in refrigerator.
Shipping Instructions:Ship at refrigerated temperature.
Stability:8 hours at room temperature; 2 weeks refrigerated; 1 year frozen.
Test Performed at or Referral Lab Lab Sendouts  (ARUP)
Referral Lab number:55260


Interpretive

Reference Range:

Negative;

IgG Positive

Any five of the following ten bands: 18, 23, 28, 30, 39, 41, 45, 58, 66, or 93 kDa.

IgG Negative

Any pattern that does not meet the IgG positive criteria.

IgM Positive

Any two of the following three bands: 23, 39, 41 kDa.

IgM Negative

Any pattern that does not meet the IgM positive criteria.

A negative result demonstrates no antibodies unique to Borrelia burgdorferi, and therefore is not supportive of Lyme disease.

A positive result is consistent with the presence of antibody produced by patients in response to infection by Borrelia burgdorferi and suggests presence of Lyme disease. Although the test has been shown to have a high degree of reliability for diagnostic purposes, laboratory data should be correlated with clinical findings.

The detection of antibodies to Borrelia burgdorferi in cerebrospinal fluid may indicate central nervous system infection. However, consideration must be given to possible contamination by blood or transfer of serum antibodies across the blood-brain barrier.

 

 

Use:Current CDC recommendations for the serologic diagnosis of Lyme Disease are to screen with polyvalent EIA test and confirm equivocal and positive results with Immunoblot. Both IgM and IgG Immunoblots should be performed on samples less than 4 weeks after appearance of erythema migrans. Only IgG Immunoblot should be performed on samples greater than 4 weeks after the disease onset. IgM Immunoblot in the chronic stage is not recommended and does not aid in the diagnosis of neuroborreliosis or chronic Lyme disease.


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