Vitamin D, 25-Hydroxy

Abbrev Code:VITD   
Order Code:LAB535Order Name:Vitamin D Deficiency
Synonyms:25-Hydroxycholecalciferol; 25-OH-Vitamin D Total; 25 Hydroxyergocalciferol; 25-Hydroxy Vitamin D; 25-OH Vitamin D Total (D2 and D3); 25-Hydroxyvitamin D2 and D3; Vitamin D Deficiency; Vitamin D Screening
CPT Codes: 82306 x1, 82306 x1
Test Includes:25-OH vitamin D3 and 25-OH vitamin D2
Turnaround Time:Performed Mon-Fri; results are reported in 1-2 days.

Collection Instructions

Optimal Volume:2 mL
Minimum\Peds Volume:0.6 mL
Container:Red or gold (gel)
Alternate Containers: Green (lithium heparin, no gel), Green (lithium heparin, gel), Purple (EDTA), Red (no gel)
Causes for Rejection:Gross hemolysis

Processing and Shipping

Specimen Processing:Centrifuge within 2 hours of collection and aliquot 1 mL, 0.2 mL minimum. Specimens collected in a green (lithium heparin or sodium heparin) tube must be centrifuged for at least five minutes before aliquoting or assaying sample.
Shipping Instructions:Ship at refrigerated temperature.
Stability:72 hours at room temperature; 12 days refrigerated; 1 year frozen.
Test Performed at or Referral Lab UM Specialty Core/Prot/Endo (Architect A)  (UMMC East Bank)


Reference Range:20-75 ug/L Season, race and dietary intake and treatment affect the concentration of 25-hydroxy-Vitamin D. Values may decrease during winter months and increase during summer months. Values 20-29 ug/L may indicate Vitamin D insufficiency and values less than 20 ug/L may indicate Vitamin D deficiency.
Therapeutic Range:20-75 ng/mL
Use:Vitamin D testing is not covered by government payers for screening. This test does not need to be ordered in combination with 1,25 Dihydroxy Vitamin D. This test measures the combined total of both 25-OH vitamin D3 and 25-OH vitamin D2 and reports the combined total. Recovery to 25-OH Vitamin D2 is only about 80%. If an individual is on Vitamin D2 (ergocalciferol) supplementation, order VITD23 by LCMSMS (LAB585).
This test is indicated for patients with chronic kidney disease, cirrhosis, hypocalcemia, hypercalciuria, hypervitaminosis D, parathyroid disorders, malabsorption states, obstructive jaundice, osteomalacia, osteoporosis, osteosclerosis/petrosis, rickets and vitamin D deficiency on replacement therapy related to a condition listed previously, to monitor the efficacy of treatment.

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