Anaplasma, Ehrlichia, Babesia Smears

Sunquest Code:PSTN  
Epic Code:LAB3697Epic Name:Parasite Stain
Methodology:Giemsa stain
Test Includes:Microscopic exam of thick and thin peripheral blood smears.
Turnaround Time:Performed routinely daily; 0730-1530. Results are reported within 24 hours of recept.
Contact the Infectious Diseases Laboratory and Medical Director for STAT testing at other times.
Special Instructions:
For STATs, the laboratory must be notified. Indicate clinical history on the request form.
  • Specify parasite suspected.
  • Provide a brief description of symptoms.
  • Indicate clinical and travel history on request form if not available in the electronic medical record.
Also see Anaplasma, Ehrlichia Antibodies

Collection Instructions

Specimen:Thick and thin blood smears prepared as for malaria AND whole blood
Optimal Volume:3 thick and 3 thin blood smears AND 2 mL blood or 0.5 mL in a microtainer. (Do not fill with more then 0.5 mL)
Minimum\Peds Volume:3 thick and 3 thin blood smears AND 1mL blood or 0.3 mL in a microtainer. (Do not fill with more than 0.5 mL)
Container:Purple (EDTA), Microscope slides
Collection Instructions:Collect purple (EDTA) tube. Immediately mix 10 times by gentle inversion. Collect blood in syringe after all other tubes are collected and immediately make thick and thin blood smears at the bedside. Alternatively, thick and thin smears can be made directly from fingerstick.
Thin smears should have long feathered edges similar to those used to study cell morphology in Hematology. Thick smears are dime sized preparations and several layers thick. See separate collection procedure "Blood Films for Malaria".
Blood smears made from EDTA tubes are inferior and only acceptable if there is no alternative. If EDTA is used, smears should be made within 2 hours of collection.
Label each slide and allow to dry flat before placing in slide holders and delivering to IDDL.  

Processing and Shipping

Specimen Processing:Store at room temperature.
Test Performed at or Referral Lab Infectious Diseases Diagnostic Laboratory  (UMMC-East Bank)


Critical Range:Positive result.
Reference Range:Negative.

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