Blastomyces dermatitidis Antigen EIA, Blood
| Sunquest Code: | BLASTB | ||
| Epic Code: | LAB6853 | Epic Name: | Blastomyces AGN Quant EIA Blood |
| Methodology: | Enzyme immunoassay | ||
| Turnaround Time: | Specimens are sent to the reference laboratory Mon-Thur; results are reported within 3-4 days. | ||
| Special Instructions: | To assess the course of antigen clearance in patients with positive results or with known blastomycosis, obtain follow-up specimens at least 14 days after the last prior specimen. | ||
Collection Instructions
| Specimen: | Blood |
| Optimal Volume: | 4 mL |
| Minimum\Peds Volume: | 2.6 mL |
| Container: | Red or gold (gel) [Alternate Containers: Green (lithium heparin, gel), Purple (EDTA), Red (no gel)] |
| Causes for Rejection: | Tissue, sputum, bronchial brushings, stool FNA, biopsy, tracheal or bone marrow aspirate, samples in transport media, fixative or isolator tubes. Samples too viscous to pipet. |
Processing and Shipping
| Specimen Processing: | Centrifuge and aliquot 2 mL, 1.2 mL minimum. Store in refrigerator. |
| Shipping Instructions: | Ship at refrigerated temperature. |
| Stability: | 14 days room temperature or refrigerated; indefinitely frozen. |
| Test Performed at or Referral Lab | Lab Sendouts (MiraVista Diagnostics) |
| Referral Lab number: | 316 |
Interpretive
| Reference Range: | None detected |
| Limitations: | Heterophile antibodies and rheumatoid factor can cause positive interference. Cross reactions due to closely related antigenic epitopes are seen in patients with histoplasmosis, paracoccidiodomycosis, and pencilliosis. Rare cross reactions have been seen in patients with aspergillosis and cryptococcosis. |
| Use: | Aid in the diagnosis of disseminated or acute pulmonary blastomycosis. |
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