BCR-ABL1: Molecular Detection of Major Breakpoint Region, p210, Quantitative

Abbrev Code:BCRQMD   
Order Code:LAB4751Order Name:BCR ABL1 Major Breakpoint Quant CML
Synonyms:Philadelphia Chromosome, CML by Molecular Detection; Real-Time RTPCR, p210; BCR ABL1; BCRCMLQT
Methodology:Real-time PCR
CPT Codes: 81206 x1, G0452 x1
Turnaround Time:Results are reported in 5-7 days.

Collection Instructions

Specimen:Blood or bone marrow or RNA
Optimal Volume:10 mL blood; 5 mL bone marrow
Minimum\Peds Volume:5 mL blood, 1 mL bone marrow; 25 uL RNA at a concentration of 20 ng/uL
Container:Yellow (ACD, Solution A) tube available from laboratory
Alternate Containers: Purple (EDTA)
Causes for Rejection:Blood or bone marrow specimen received more than 72 hours from the date of collection; incorrect volume or tube type; clotted or frozen specimens; RNA not shipped on dry ice. RNA extracted at non-CLIA certified (or equivalent) LAB.
Shared samples will not be accepted unless sent to Molecular first. Contamination will occur on automated hematology analyzers.

Processing and Shipping

Specimen Processing:Store blood or bone marrow in refrigerator. Store RNA on dry ice.
Shipping Instructions:Ship blood or bone marrow at refrigerated temperature to arrive within 24 hours of collection, Mon-Fri only. See Cause for Rejection. Ship RNA on dry ice. If shipping on Thursday or Friday, contact lab at 612-273-8445.
Test Performed at or Referral Lab UM Molecular Diagnostics (M)  (UMMC East Bank)


Reference Range:See interpretive report.
Use:Chromosomal translocation (9;22) leads to a fusion gene BCR-ABL1. The fusion gene is a signature feature of the Chronic Myeloproliferative disorder (CML). During and after treatment, patients with CML can be monitored for presence or absence of BCR-ABL1 fusion transcripts. Quantitative RT-PCR can provide important information to monitor the tumor load, status of remission, the minimal residual disease, and chronological expression level of the fusion transcripts. This test does not include BCR/ABL1 fusion transcripts, p190 (minor breakpoint of BCR-ABL1), for ALL. Follow-up testing for BCR-ABL1 CML should be done on same specimen type, preferably blood. Bone marrow samples will be accepted, but comparisons between blood and bone marrow may not be appropriate.

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