Cortisol, Urine, Free by LC-MS/MS


Sunquest Code:UCORTF  
Epic Code:LAB568Epic Name:Cortisol Free Urine
Synonyms:17-Ketogenic Steroids, Urine; Free Cortisol, Urine
Methodology:Liquid chromatography/Tandem mass spectrometry
Turnaround Time:Specimens are sent to reference laboratory Mon-Sat; results are reported within 1-4 days.
Compliance:

This test was developed and its performance characteristics determined by ARUP Laboratories. It has not been cleared or approved by the U.S. Food and Drug Administration. This test was performed in a CLIA certified laboratory and is intended for clinical purposes.
Associated Links:

Urine Collection Procedure


Collection Instructions

Specimen:Urine, 24 hour collection or random
Optimal Volume:Submit entire 24 hour collection; analysis requires 1 mL
Container:3 L plastic jug
[Alternate Containers: Plastic leakproof container, sterile]
Collection Instructions:Refrigerate during collection.
Causes for Rejection:Room temperature specimens. Acidified specimens or specimens with preservatives.


Processing and Shipping

Specimen Processing:Mix collection well before aliquot is taken. Aliquot 4 mL, 1 mL minimum. Store in refrigerator. Record total volume and collection time interval on transport tube and test request form.
Shipping Instructions:Ship at refrigerated temperature.
Stability:Stability: 2 weeks refrigerated; 6 months frozen
Test Performed at or Referral Lab Lab Sendouts  (ARUP)
Referral Lab number:97222


Interpretive

Reference Range:
Cortisol (µg/day):
Age Gender Reference Interval
3-8y F & M Less than or equal to 18 µg/d
9-12 y F & M Less than or equal to 37 µg/d
13-17 y F & M Less than or equal to 56 µg/d
18 y& older F Less than or equal to 45 µg/d
18 y & older M Less than or equal to 60 µg/d

Cortisol (µg/g crt):
Age Gender Reference Interval
Prepubertal F Less than or equal to 25 µg/g crt
18 y& older F Less than or equal to 24 µg/g crt
Pregnancy F Less than or equal to 59 µg/g crt
Prepubertal M Less than or equal to 25 µg/g crt
18 y & older M Less than or equal to 32 µg/g crt
The optimal specimen for this testing is a 24 hour urine collection. Mass per day calculations are not reported for the following specimen types: a random collection, a collection with duration of less than 20 hours, a collection with a duration of greater than 28 hours, or a collection with a total volume less than 400 mL or greater than 5000 mL. Ratios to creatinine may be useful for these evaluations. 


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