Treponema Antibody with Reflex to RPR and Titer

Abbrev Code:TREPT   
Order Code:LAB8011Order Name:Treponema Antibody with Reflex to RPR and titer
Synonyms:Syphilis Antibody; Treponema Pallidum Antibody; STI; RPR; STD; TREPAB; Anti-Treponema
Methodology:Chemiluminescent immunoassay
CPT Codes: 86780, 0064U x1, 86780, 0064U x1, 86592, 0065U x1, 86592, 0065U x1, 86593 x1, 86593 x1, 86780 x1, 86780 x1
Turnaround Time:Performed daily; results are reported within 24 hours.
Associated Links:

Syphilis Serology Algorithm

Collection Instructions

Optimal Volume:6 mL
Minimum\Peds Volume:4.2 mL (See Collection Instructions for pediatric patients.)
Container:Red or gold (gel)
Alternate Containers: Red (no gel)
Collection Instructions:Minimum volume is 0.9 mL for pediatric patients only. If positive, the Rapid Plasma Reagin with Reflex to Titer (RPRAB) will need to be ordered and 4.2 mL additional sample acquired.
Causes for Rejection:Hemolysis
Contraindications:This test (TREPT) is not used to test CSF specimens; see VDRL, Cerebrospinal.

Processing and Shipping

Specimen Processing:Centrifuge and leave sample in serum separator tube (preferred). Alternately, aliquot 3 mL, 2 mL minimum. (0.35 mL for pediatric patients only. If positive, the Rapid Plasma Reagin with Reflex to Titer (RPRAB) will need to be ordered and 2.0 mL additional aliquot is required.) Store refrigerated.
Shipping Instructions:Ship at refrigerated temperature.
Stability:7 days at refrigerated temperature; for longer storage freeze.
Test Performed at or Referral Lab UM Specialty Core/Prot/Endo (Liaison D) 


Reference Range:
Use:Use for screening for exposure to Treponema pallidum. The Anti-Treponema screening test tends to remain positive for life, and therefore does not distinguish between active and post syphilis infections. All positive and equivocal results will automatically reflex to a non-specific Rapid Plasma Reagin (RPRAB) test. If the Anti-Treponema is positive, and the RPR is reactive, this is presumptive evidence of current infection, inadequately treated infection, persistent infection or reinfection. If the Anti-Treponema is positive and the RPR is negative, then a second Treponema specific test, Treponema pallidum particle agglutination (TP-PA) will be performed to determine whether the anti-Treponema antibody is falsely positive or is detecting early infection. If the latter is suspected, repeat testing in approximately 2 weeks is recommended.

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