HIV-1 RNA Quantitation

Abbrev Code:HIVPCR   
Order Code:LAB878Order Name:HIV-1 RNA Quantitative
Synonyms:HIV Quantitation; HIV Viral Load; HIV 1 RNA
Methodology:Real-Time Reverse Transcription Polymerase Chain Reaction (RT-PCR)
CPT Codes: 87536 x1
Turnaround Time:Testing is performed 2 days/week; results are reported within 4 days.

Collection Instructions

Optimal Volume:8 mL
Minimum\Peds Volume:4 mL
Container:10 mL Purple EDTA tube
Causes for Rejection:Heparinized and serum specimens are not acceptable for testing.

Processing and Shipping

Specimen Processing:Within 24 hours, centrifuge and aliquot 2 mL, 1.2 mL minimum plasma. Store in refrigerator.
Shipping Instructions:Ship at refrigerated or frozen.
Stability:Plasma is stable for 6 days at refrigerated temperature; 84 days frozen.
Test Performed at or Referral Lab Infectious Diseases Diagnostic Laboratory   (UMMC East Bank)


Reference Range:Not detected.
This assay has a plasma HIV-1 RNA quantification result range of 20 to 10,000,000 copies/mL (1.3 to 7.0 log copies/mL).
Result Indicates
Not Detected The assay was unable to detect HIV-1 RNA within the plasma specimen.
<20 copies/mL
(<1.3 log copies/mL)
HIV-1 RNA is detected, but the level present is less than the lower quantification limit of the assay.
>10,000,000 copies/mL
(>7.0 log copies/mL)
HIV-1 RNA is detected, but the level present is above the upper quantification limit of the assay.
Use:This assay is intended for use in conjunction with clinical presentation and other laboratory markers for disease prognosis and for use as an aid in assessing viral response to antiretroviral treatment as measured by changes in plasma HIV-1 RNA levels. This test should not be used as a donor screening test for HIV-1 or as a diagnostic test to confirm the presence of HIV-1 infection.

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