Clostridioides (Clostridium) difficile Toxin B PCR

Abbrev Code:CDBPCR   
Order Code:LAB4565Order Name:Clostridium difficile Toxin B PCR
Synonyms:Cdiff; Stool; C Diff; Cdiff Toxin; CDBPCR
Methodology:Real-time polymerase chain reaction (PCR)
CPT Codes: 87493 x1
Turnaround Time:Performed daily; results are reported within 24 hours.
Associated Links:

IDDL Stool Collection Container Guidelines

Collection Instructions

Specimen:Feces, liquid/pourable/unformed/conforms to shape of container
Optimal Volume:3 mL
Minimum\Peds Volume:0.5 mL
Container:Plastic Leakproof Container or Sterile Stool Transport Vial
Collection Instructions:Only one specimen accepted per 24 hour period.
Causes for Rejection:Formed and semi-formed stools are not acceptable. Stool samples must be liquid/pourable and unformed in consistency. Rectal swabs are not acceptable. Feces collected in preservative are not acceptable.
Contraindications:Per assay package insert: Inhibition of the c. difficule assay has been observed in the presence of the following substances: zinc oxide paste and Vagisil cream. Performance characteristics were not established for patients less than 2 years of age with this assay. The effects of urine contamination in stool specimens submitted for c. difficile PCR testing are unknown. Therefore, it is advisable to avoid urine contamination.

Processing and Shipping

Specimen Processing:Store in refrigerator.
Shipping Instructions:Ship at refrigerated temperature. Must arrive within 5 days.
Test Performed at or Referral Lab Infectious Diseases Diagnostic Laboratory  (Grand Itasca, Range, UMMC East Bank)


Reference Range:
Negative for C. difficile toxin B by PCR  
Use:Detect C. difficile toxin B in patients experiencing loose, watery stools with risk factors (e.g. recent antibiotic use, immunosuppression, hospital stay).

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