T Helper/Inducer:T Suppressor Cytotoxic Lymphocyte Ratio, B Cell and Natural Killer Cells

Abbrev Code:THTSXB   
Order Code:LAB3245Order Name:T Cell Subset Extended Profile
Synonyms:CD4 Count; CD4:CD8 Lymphocyte Ratio; T Cell Subsets; T4:T8 Lymphocyte Ratio; NK Cells; T and B Cell Quantitation; Lymphocyte Subset Analysis; THTSX; B Cells, Absolute CD4
Methodology:Multi-color flow cytometry
CPT Codes: 86359 x1, 86360 x1, 86355 x1, 86357 x1
Test Includes:Total T lymphocytes and subset enumeration, B cell enumeration and NK cell enumeration, which includes percentages, absolute values per cu mm, T helper: T suppressor ratio.
Turnaround Time:Testing is performed Mon-Fri 08:00-16:00 and on specimens arriving in the Flow Cytometry lab on Saturday before noon. No testing is performed on Saturdays after noon, Sundays, or holidays. Results are reported within 1-2 business days.
Special Instructions:Provide diagnosis, age, collection date, and time. Samples must be received and tested within 24 hours of collection. Samples will be tested Mon-Fri 08:00-16:00 and on Saturday before noon. Samples are received in the Core lab when the Flow Cytometry lab is not staffed. Contact the IFC laboratory at 612-273-5248 for further information
Associated Links:

IFC Reference Intervals

Collection Instructions

Optimal Volume:4 mL
Minimum\Peds Volume:0.5 mL
Container:Purple (EDTA)
Collection Instructions:
Samples must be maintained at room temperature. Samples must be less than 24 hours old at the time of testing and are received in the Flow Cytometry laboratory Monday-Friday and until noon on Saturday. Samples are received in the Core lab when the Flow Cytometry lab is not staffed. Specimens will be tested the next day of lab operation if less than 24 hours old. Samples are not tested on holidays. Contact the IFC laboratory at 612-273-5248 for further information.  
Causes for Rejection:Blood with low WBC and/or percentage of lymphocytes, clotted specimen, and specimens greater than 24 hours old. Refrigerated specimens.

Processing and Shipping

Specimen Processing:Do not centrifuge. Store at room temperature. Send to Flow Cytometry lab within 24 hours of collection.
Shipping Instructions:Ship at room temperature to arrive within 24 hours of collection. Do not send on weekends or the day before a holiday.
Test Performed at or Referral Lab UM Flow Cytometry (W)  (UMMC East Bank)


Reference Range:


See Associated Links for Reference Intervals.


Use:Diagnosis and monitoring of acquired immunodeficiency syndrome (HIV infection).

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