HLA Auto Crossmatch, Recipient
| Sunquest Code: | ATOXMR | ||
| Epic Code: | LAB6470 | Epic Name: | HLA Auto Crossmatch |
| Order Instructions: | For pediatric or difficult collection, call Immunology 612-273-3100 for additional minimum volume information. | ||
| Synonyms: | Auto-Flow Crossmatch; T and B Lymphocyte, Flow Crossmatch | ||
| Methodology: | Flow cytometry | ||
| Test Includes: | Selected patient sera are crossmatched against allogeneic and autologous T and B lymphocytes. | ||
| Turnaround Time: | Results are reported within 5 days. | ||
| Special Instructions: | Deliver directly to Immunology Laboratory (7-139 PWB) immediately after collection. If this test is being added on, comment ADD-ON in the Comment field when placing a normal order in Epic. Immunology will receive the printout directly. All other lab areas should discard the Immunology ADD-ON printout. | ||
Collection Instructions
| Specimen: | Blood |
| Optimal Volume: | 14 mL red (plain) tube AND 40 mL Yellow (ACD, Solution A) tube |
| Minimum\Peds Volume: | 3 mL red (plain) tube AND 10 mL Yellow (ACD, Solution A) tube |
| Container: | Red (no gel), Yellow (ACD, Solution A) tube available from laboratory (Red (gel)) |
| Collection Instructions: | Collect specimen prior to dialysis. Inform lab if patient is receiving Rituxamib. |
| Causes for Rejection: | ACD: Clotted specimen.
Red: Gross hemolysis, immunosuppression, drug interference |
| Contraindications: | Serum will not clot (if collected during or immediately after dialysis). |
Processing and Shipping
| Specimen Processing: | Store at room temperature. |
| Shipping Instructions: | Ship at room temperature. Testing must be performed within 3 days of collection. Do not send routine testing on Friday. |
| Test Performed at or Referral Lab | Immunology (UMMC-East Bank) |
Interpretive
| Use: | Determine presence or absence in potential recipient's serum of HLA antibody reactive with potential donor's cells which may result in hyperacute rejection of a transplant. |
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