HLA Auto Crossmatch, Recipient
Abbrev Code: | ATOXMR | ||
Order Code: | LAB6470 | Order Name: | HLA Auto Crossmatch |
Order Instructions: | For pediatric or difficult collection, call Immunology 612-273-3100 for additional minimum volume information. | ||
Synonyms: | Auto-Flow Crossmatch; T and B Lymphocyte, Flow Crossmatch | ||
Methodology: | Flow cytometry | ||
CPT Codes: | 86825 x1 | ||
Test Includes: | Selected patient sera are crossmatched against allogeneic and autologous T and B lymphocytes. | ||
Turnaround Time: | Results are reported within 5 days. | ||
Special Instructions: | Deliver directly to Immunology Laboratory (7-139 PWB) immediately after collection. If this test is being added on, comment ADD-ON in the Comment field when placing a normal order in Epic. |
Collection Instructions
Specimen: | Blood |
Optimal Volume: | 14 mL red (plain) tube AND 20 mL Yellow (ACD, Solution A or ACD, Solution B) tube |
Minimum\Peds Volume: | 3 mL red (plain) tube AND 10 mL Yellow (ACD, Solution A or ACD, Solution B) tube |
Container: | Red (no gel), Yellow (ACD, Solution A) tube available from laboratory Alternate Containers: Red (gel), Yellow (ACD, Solution B) tube available from laboratory |
Collection Instructions: | Collect specimen prior to dialysis. Inform lab if patient is receiving Rituxamib. |
Causes for Rejection: | ACD: Clotted specimen.
Red: Gross hemolysis, immunosuppression, drug interference |
Contraindications: | Serum will not clot (if collected during or immediately after dialysis). |
Processing and Shipping
Specimen Processing: | Store at room temperature. |
Shipping Instructions: | Ship at room temperature. Testing must be performed within 3 days of collection. Do not send routine testing on Friday. |
Test Performed at or Referral Lab | Immunology (UMMC East Bank) |
Interpretive
Use: | Determine presence or absence in potential recipient's serum of HLA antibody reactive with potential donor's cells which may result in hyperacute rejection of a transplant. |
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