Vitamin B12

Abbrev Code:VB12   
Order Code:LAB67Order Name:Vitamin B12
Synonyms:True Cobalamin
Methodology:Electrochemiluminescent immunoassay (ECLIA)
CPT Codes: 82607 x1, 82607 x1
Turnaround Time:Performed and reported 24 hours/day.

Collection Instructions

Optimal Volume:2.5 mL
Minimum\Peds Volume:0.6 mL
Container:Green (lithium heparin, gel)
Alternate Containers: Green (lithium heparin, no gel), Red (no gel), Red (gel)
Patient Preparation:Fasting specimen is preferred.
Please do not eat 10-12 hours before your appointment if you are coming in fasting for labs on lipids, cholesterol, or glucose (sugar). This does not apply to pregnant women.
Water, hot tea and black coffee (with nothing added ) is okay. Do not drink other fluids, diet soda or chew gum.
If you have concerns about taking your medications, please send a message by clicking on Secure Messaging, Message Your Care Team.
Baseline level must be collected before Schilling test, transfusions or B12 therapy is started.
Causes for Rejection:Hemolyzed specimen

Processing and Shipping

Specimen Processing:Centrifuge and aliquot 1.25 mL, 0.2 mL minimum within 2 hours of collection. Store in refrigerator up to 48 hours; otherwise, freeze.
Shipping Instructions:Ship at refrigerated temperature to arrive within 48 hours.
Stability:2 hours at room temperature; 48 hours refrigerated; 56 days frozen. Freeze only once.
Test Performed at or Referral Lab Chemistry  (Roche-Grand Itasca, Roche-UMMC East)


Reference Range:Ages 22-79: 232-1245 pg/mL
Use:Evaluation of anemia.

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