Vitamin B12


Abbrev Code:VB12   
Order Code:LAB67Order Name:Vitamin B12
Synonyms:True Cobalamin
Methodology:Chemiluminscent immunoassay
St Joseph: CMIA
CPT Codes: 82607 x1
Turnaround Time:Performed and reported 24 hours/day.


Collection Instructions

Specimen:Blood
Optimal Volume:2.5 mL
Minimum\Peds Volume:0.6 mL
Container:Green (lithium heparin, gel), St. Joseph - Red (no gel)
Alternate Containers: Green (lithium heparin, no gel), Purple (EDTA), Red (no gel), Red or gold (gel)
Patient Preparation:Fasting specimen is preferred.
Please do not eat 10-12 hours before your appointment if you are coming in fasting for labs on lipids, cholesterol, or glucose (sugar). This does not apply to pregnant women.
Water, hot tea and black coffee (with nothing added ) is okay. Do not drink other fluids, diet soda or chew gum.
If you have concerns about taking your medications, please send a message by clicking on Secure Messaging, Message Your Care Team.
Baseline level must be collected before Schilling test, transfusions or B12 therapy is started.
Causes for Rejection:Hemolyzed specimen


Processing and Shipping

Specimen Processing:Centrifuge and aliquot 1.25 mL, 0.2 mL minimum within 2 hours of collection. Store in refrigerator up to 48 hours; otherwise, freeze.
Shipping Instructions:Ship at refrigerated temperature to arrive within 48 hours. Alternatively, ship on dry ice.
Stability:24 hours at room temperature; 48 hours refrigerated; indefinitely frozen.
Test Performed at or Referral Lab Chemistry  (UMMC East, CSC Mpls, Grand Itasca, St. Joseph's)


Interpretive

Reference Range:Grand Itasca: 180-914 pg/mL
St Joseph: 213-816 pg/mL
All others: 193-986 pg/mL
Use:Evaluation of anemia.


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