Vitamin B12

Sunquest Code:VB12  
Epic Code:LAB67Epic Name:Vitamin B12
Synonyms:True Cobalamin; Vit B12; VitB12; VB12
Methodology:Chemiluminescent immunoassay
Turnaround Time:Performed and reported 24 hours/day.

Collection Instructions

Optimal Volume:2.5 mL
Minimum\Peds Volume:0.6 mL
Container:Red or gold (gel) (Green (lithium heparin, gel), Green (lithium heparin) on ICE, Green (sodium heparin, no gel), Green (sodium heparin, no gel) on ICE, Purple (EDTA), Red (no gel))
Patient Preparation:Fasting specimen is preferred.
Please do not eat 10-12 hours before your appointment if you are coming in fasting for labs on lipids, cholesterol, or glucose (sugar). This does not apply to pregnant women.
Water, hot tea and black coffee (with nothing added ) is okay. Do not drink other fluids, diet soda or chew gum.
If you have concerns about taking your medications, please send a message by clicking on Secure Messaging, Message Your Care Team.
Baseline level must be collected before Schilling test, transfusions or B12 therapy is started.
Causes for Rejection:Hemolyzed specimen

Processing and Shipping

Specimen Processing:Centrifuge and aliquot 1.25 mL, 0.2 mL minimum within 2 hours of collection. Store in refrigerator up to 48 hours; otherwise, freeze.
Shipping Instructions:Ship at refrigerated temperature to arrive within 48 hours. Alternatively, ship on dry ice.
Stability:Stability: Room temperature - 24 hours; refrigerated - 48 hours; frozen - indefinitely.
Test Performed at or Referral Lab Chemistry  (UMMC-East Bank, M Health Clinic and Surgery Center, Grand Itasca)


Reference Range:193-986 pg/mL
GICH: 180-914 pg/mL  
Use:Evaluation of anemia.

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