Cytotoxic T-Lymphocyte

Abbrev Code:XMISC   
Order Code:LAB4909Order Name:Laboratory Miscellaneous Order
Synonyms:CTL Function; Cytotoxic T Lymphocyte
Methodology:Chromium release
CPT Codes: 86352 x1
Turnaround Time:Specimens are sent to reference laboratory Mon-Thur by 1500; results are reported within 14 days.
Special Instructions:A completed requisition and genetic consent form must accompany each sample. See Associated Links. Samples must be received in testing laboratory within 24 hours of collection.

Order On Time Delivery courier to deliver directly to East Bank Sendouts Lab. Call Sendouts at 612-273-3711 to notify.

CSC and West only: Send in red STAT bag by HealthEx courier. Call Sendouts at 612-273-3711 to notify.
Associated Links:

Genetic Testing Consent Form, Customized Volumes by ALC, Cincannati Children's Hospital Requisition Form

Collection Instructions

Optimal Volume:10 mL
Container:Green (sodium heparin, no gel)
Collection Instructions:Specimens must be collected after 10:00 am  and received in East Bank Core Lab by 1400, Monday through Thursday. Do not collect the day before a holiday.

Volumes requested assume abnormal absolute lymphocyte count (ALC). If the ALC is abnormal, use the Customized Volumes by ALC information to obtain correct volume, See Associated Links.
Causes for Rejection:Samples received in testing laboratory greater than 24 hours old.

Processing and Shipping

Specimen Processing:Whole blood. Do not process.
Shipping Instructions:Ship whole blood in original container at room temperature. Samples must be received in testing laboratory within 24 hours of collection and before 11:00 am on Fridays. Protect from temperature extremes. Call CBDI DIL at 513-636-4685 with courier and sample tracking number.
Stability:24 hours at room temperature.
Test Performed at or Referral Lab Lab Sendouts  (Cincinnati Children's Hospital Medical Center)
Referral Lab number:2900650


Reference Range:By report.
Use:Cytotoxic T lymphocytes (CTL) are a subset of lymphocytes that have the ability to lyse target cells bearing specific antigens. To assess the functionality of CTL's, target cells previously primed to contain radioactive chromium within the cell membrane, are incubated with patient or control lymphocytes. Lysis of the target cells by the CTL's is measured by the amount of chromium released into the supernatant.

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