WBC Differential Count


Abbrev Code:WDIFF   
Order Code:LAB3880Order Name:Differential
Order Instructions:Must be ordered with WBC (LAB299) or CBC (LAB294)
Synonyms:Differential Leukocyte Count; Leukocyte Differential Count
CPT Codes: 85004 x1
Test Includes:Relative frequency (%) and absolute value of the white blood cells. Test may include manual leukocyte differential, RBC morphology and/or PLT evaluation if reflex criteria is met.
Turnaround Time:Performed and reported 24 hours/day.
Special Instructions:A WBC must be ordered with every differential.


Collection Instructions

Specimen:Blood
Optimal Volume:2-3 mL EDTA tube or 0.5 mL in an EDTA microtainer (do not fill more than 0.5 mL)
Minimum\Peds Volume:1.0 mL EDTA tube or 0.3 mL in an EDTA microtainer
Container:Purple (EDTA)
Collection Instructions:Immediately mix specimen 10 times by gentle inversion.
Causes for Rejection:Clotted specimen.


Processing and Shipping

Specimen Processing:Store in refrigerator.
Shipping Instructions:Ship at refrigerated temperature. If more than 24 hours is required for transport, 2 unstained whole blood smears must accompany whole blood EDTA specimen.
Test Performed at or Referral Lab Hematology  (Clinics, CSC Mpls, Grand Itasca, Lakes, Northland, Range, Ridges, Southdale, St. John's, UMMC East Bank, UMMC/UMMCH-West Bank, Woodwinds)


Interpretive

Critical Range:
Absolute Neutrophil: Less than 0.5 (x 109/L)  
Reference Range:

 

Absolute Diff Value (x 109/L)

 

Age

Neut

Lymph

Mono

Eos

Baso

IG

0-7 d

2.9-26.6

1.7-12.9

0-1.1

0-0.7

0-0.2

0-1.8

8-13 d

1.0-12.8

1.3-11.1

0-1.1

0-0.7

0-0.2

0-1.8

14-30 d

1.0-12.8

1.3-11.1

0-1.1

0-0.7

0-0.2

0-1.3

1-11 mo

1.0-12.8

2.0-14.9

0-1.1

0-0.7

0-0.2

0-0.8

1-5 y

0.8-7.7

2.3-13.3

0-1.1

0-0.7

0-0.2

0-0.8

6-9 y

1.3-8.1

1.1-8.6

0-1.1

0-0.7

0-0.2

0-0.4

10-17 y

1.3-7.0

1.0-5.8

0-1.3

0-0.7

0-0.2

0-0.4

18 y & older

1.6-8.3

0.8-5.3

0-1.3

0-0.7

0-0.2

0-0.4

 

Leukocyte Differential Count

Relative Frequency (%)

 

Age

Neut

Lymph

Mono

Eos

Baso

IG

0-7 d

32-76

19-36

0-10

0-6

0-1

0-0.28

8-13 d

19-61

26-53

0-10

0-6

0-1

0-0.28

14-30 d

19-61

26-53

0-10

0-6

0-1

0-0.22

1-11 mo

19-61

41-71

0-10

0-6

0-1

0-0.09

1-5 y

15-44

45-76

0-10

0-6

0-1

0-0.09

6-9 y

32-54

27-57

0-10

0-6

0-1

0-0.03

10-17 y

32-64

26-50

0-12

0-6

0-2

0-0.03

18 y & older

40-75

20-48

0-12

0-6

0-2

0-0.03

 

 

 

Use:Evaluate and monitor hematologic disorders and drug, radiation, bone marrow transplant therapy; with WBC count, calculate absolute numbers of leukocyte types; help distinguish bacterial and viral infections.


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