WBC Differential Count


Sunquest Code:WDIFF  
Epic Code:LAB3880Epic Name:Differential
Order Instructions:Must be ordered with WBC (LAB299) or CBC (LAB294)
Synonyms:Differential Leukocyte Count; Leukocyte Differential Count
Test Includes:Relative frequency (%) and absolute value of the white blood cells. Test may include manual leukocyte differential, RBC morphology and/or PLT evaluation if reflex criteria is met.
Turnaround Time:Performed and reported 24 hours/day.
Special Instructions:A WBC must be ordered with every differential.


Collection Instructions

Specimen:Blood
Optimal Volume:2-3 mL EDTA tube or 0.5 mL in an EDTA microtainer (do not fill more than 0.5 mL)
Minimum\Peds Volume:1.0 mL EDTA tube or 0.3 mL in an EDTA microtainer
Container:Purple (EDTA)
Collection Instructions:Immediately mix specimen 10 times by gentle inversion.
Causes for Rejection:Clotted specimen.


Processing and Shipping

Specimen Processing:Store in refrigerator.
Shipping Instructions:Ship at refrigerated temperature. If more than 24 hours is required for transport, 2 unstained whole blood smears must accompany whole blood EDTA specimen.
Test Performed at or Referral Lab Hematology  (Range, UMMC-East Bank, Lakes, Ridges, Northland, Southdale, UMMC/UMMCH-West Bank, Clinics, M Health Clinic and Surgery Center, Grand Itasca, St. Joseph's, St. John's, Woodwinds)


Interpretive

Critical Range:
Absolute Neutrophil: Less than 0.5 (x 109/L)  
Reference Range:

 

Absolute Diff Value (x 109/L)

 

Age

Neut

Lymph

Mono

Eos

Baso

IG

0-7 d

2.9-26.6

1.7-12.9

0-1.1

0-0.7

0-0.2

0-1.8

8-13 d

1.0-12.8

1.3-11.1

0-1.1

0-0.7

0-0.2

0-1.8

14-30 d

1.0-12.8

1.3-11.1

0-1.1

0-0.7

0-0.2

0-1.3

1-11 mo

1.0-12.8

2.0-14.9

0-1.1

0-0.7

0-0.2

0-0.8

1-5 y

0.8-7.7

2.3-13.3

0-1.1

0-0.7

0-0.2

0-0.8

6-9 y

1.3-8.1

1.1-8.6

0-1.1

0-0.7

0-0.2

0-0.4

10-17 y

1.3-7.0

1.0-5.8

0-1.3

0-0.7

0-0.2

0-0.4

18 y & older

1.6-8.3

0.8-5.3

0-1.3

0-0.7

0-0.2

0-0.4

 

Leukocyte Differential Count

Relative Frequency (%)

 

Age

Neut

Lymph

Mono

Eos

Baso

IG

0-7 d

32-76

19-36

0-10

0-6

0-1

0-0.28

8-13 d

19-61

26-53

0-10

0-6

0-1

0-0.28

14-30 d

19-61

26-53

0-10

0-6

0-1

0-0.22

1-11 mo

19-61

41-71

0-10

0-6

0-1

0-0.09

1-5 y

15-44

45-76

0-10

0-6

0-1

0-0.09

6-9 y

32-54

27-57

0-10

0-6

0-1

0-0.03

10-17 y

32-64

26-50

0-12

0-6

0-2

0-0.03

18 y & older

40-75

20-48

0-12

0-6

0-2

0-0.03

 

 

 

Use:Evaluate and monitor hematologic disorders and drug, radiation, bone marrow transplant therapy; with WBC count, calculate absolute numbers of leukocyte types; help distinguish bacterial and viral infections.


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