Lymphocyte Antigen and Mitogen Proliferation


Sunquest Code:LAM  
Epic Code:LAB3580Epic Name:Lymphocyte Agn and Mitogen PROLIF Panel
Synonyms:Mitogen and Antigen Stimulation
Methodology:Cell culture
Turnaround Time:Specimens are sent to the reference laboratory Mon-Thurs; results are reported in 9-10 days.
Special Instructions:Send sample in a STAT bag to East Bank Core Lab/Sendouts:

Time Sensitive Handling Required:
Specimen must be at the East Bank Sendouts lab no later than 1600. Order On-Time Delivery courier to deliver directly to East Bank Sendouts lab. Call Sendouts at 612-273-3711 to notify.
CSC and West Only: Send in red STAT bag by HealthEx courier. Call Sendouts at 612-273-3711 to notify.
Compliance:

This test was developed and its performance characteristics determined by ARUP Laboratories. It has not been cleared or approved by the U.S. Food and Drug Administration. This test was performed in a CLIA certified laboratory and is intended for clinical purposes.



Collection Instructions

Specimen:Blood
Optimal Volume:10 mL and a 10 mL normal control from an unrelated donor
Minimum\Peds Volume:7 mL (adult) or 3 mL (pediatric) and 7 mL normal control from an unrelated donor
Container:Yellow (ACD, Solution A) tube available from laboratory (Green (sodium heparin, no gel))
Collection Instructions:Sample and control must be collected Monday through Thursday. Specimen and control must be in the original tubes and arrive at the reference laboratory within 48 hours of collection time and before noon on Friday. Testing is performed Tuesday through Friday only.
Causes for Rejection:Refrigerated or frozen samples, or samples in transport longer than 48 hours.


Processing and Shipping

Specimen Processing:Whole blood. Do not process. Send sample and control at room temperature.
Shipping Instructions:Ship at room temperature. Must arrive within 48 hours of testing and by noon on Friday. Live lymphocytes required. Critical ambient, do not refrigerate or freeze.
Test Performed at or Referral Lab Lab Sendouts  (ARUP)
Referral Lab number:96056


Interpretive

Reference Range:By report.
Use:
Phytohemagglutinin concanavalin A, pokeweed mitogen, Candida antigen and tetanus antigen are tested independently in lymphocyte culture. Lymphocyte proliferation in response to these mitogens and antigens is determined by 3H-thymidine incorporation.

Results are reported as counts per minute (CPM) mitogen or antigen stimulated versus a control culture and a Simulation Index (SI) which represents the ratio of CPM of the stimulated lymphocytes to the mean CPM of the unstimulated control.


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