Vitamin D, 25 OH D2 and D3 by LC Mass Spectrometry-Recommended for patients on ergocalciferol therapy

Abbrev Code:VITD23   
Order Code:LAB585Order Name:25 Hydroxyvitamin D2 and D3
Synonyms:25 Hydroxyergocalciferol; 25-Hydroxycholecalciferol; Fractionated 25-OH-Vitamin D Isomers
Methodology:Liquid chromatography/Tandem mass spectrometry
CPT Codes: 82306 x1
Test Includes:25-OH vitamin D2, 2-OH vitamin D3, and total 25-OH vitamin D
Turnaround Time:Performed twice per week; results are reported the next day.

Collection Instructions

Optimal Volume:2 mL
Minimum\Peds Volume:0.6 mL
Container:Red or gold (gel)
Alternate Containers: Green (lithium heparin, no gel), Green (lithium heparin, gel), Green (sodium heparin, no gel), Purple (EDTA), Red (no gel)

Processing and Shipping

Specimen Processing:Centrifuge and aliquot 1 mL, 0.2 mL minimum within 2 hours of collection. Store in refrigerator.
Shipping Instructions:Ship at refrigerated temperature. Alternatively, ship on dry ice.
Stability:72 hours at room temperature; 12 days refrigerated; 1  year frozen.
Test Performed at or Referral Lab UM Special Drug/Biochemical Genetics (Y)  (UMMC East Bank)


Reference Range:

20-75 ug/L

Season, race and dietary intake and treatment affect the concentration of 25-hydroxy-Vitamin D. Values may decrease during winter months and increase during summer months. Values 20-29 ug/L may indicate Vitamin D insufficiency and values less than 20 ug/L may indicate Vitamin D deficiency.



Use:25-OH vitamin D2, 25-OH vitamin D3 and total 25-OH vitamin D are all reported. This test is recommended only for patients receiving the prescription form of vitamin D which is vitamin D2 (ergocalciferol). All vitamin D which is naturally synthesized by the body upon sun exposure and now virtually all over-the-counter vitamin D preparations are vitamin D3 (cholecalciferol).
Vitamin D25 testing is not covered by government payers for screening. This test does not need to be ordered in combination with 1,25 Dihydroxy Vitamin D.

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