Direct Antiglobulin Test, Adult

Abbrev Code:DAT POLY   
Order Code:LAB274Order Name:Direct Antiglobulin Test
Order Instructions:SafeTrace Code: DAT_POLYG
Synonyms:Anti-Human Globulin; Coombs Test; DAT; Direct Coombs; Elution
CPT Codes: 86880 x1
Test Includes:Use of anti-IgG, C3d if test is positive, monospecific anti-IgG and monospecific anti-C3d will be used. Elution studies will be performed and charged if indicated. (UMMC-East Bank).

The provider may order LAB274 (Direct Antiglobulin Test) on both adult and neonatal patients. Epic will route this to the proper test approriate for the patient's age. Adult Direct Antiglobulin Test (PLAB2224) includes testing with polyspecific Anti-IgG, C3d.
Turnaround Time:Performed and reported 24 hours/day.

Collection Instructions

Optimal Volume:2 mL
Minimum\Peds Volume:1 mL
Container:Purple (EDTA)
Collection Instructions:Strict specimen labeling requirements must be followed for blood bank testing. The patient's full name and identification number on the specimen label MUST EXACTLY MATCH the name and identification number on the request form. The specimen label must include the following information:
  1. Patient's first and last name
  2. Patient's identification number
  3. Date and time of specimen collection
  4. Initials of the person collecting the sample, indicating that the patient's identity has been verified.

Processing and Shipping

Specimen Processing:Store at refrigerated temperature.
Shipping Instructions:Ship at refrigerated temperature.
Stability:48 hours at refrigerated temperature.
Test Performed at or Referral Lab Blood Bank  (Grand Itasca, Lakes, Northland, Range, Ridges, Southdale, St. John's, UMMC East Bank, UMMC/UMMCH-West Bank, Woodwinds)


Reference Range:By report
Use:Detects immunoproteins, IgG or complement absorbed on red cells. Used to detect autoimmune hemolytic anemias caused by antibody and/or complement components bound to patient red cells (including drug-induced), transfusion reaction, and erythroblastosis fetalis.

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