HIV Antigen/Antibody Combination
| Abbrev Code: | HIAGAB | ||
| Order Code: | LAB6327 | Order Name: | HIV Antigen Antibody Combo |
| Synonyms: | Human Immunodeficiency Virus Antigen/Antibody; HIV AG AB; HIV Screen; HIAGAB | ||
| Methodology: | Chemiluminescent immunoassay | ||
| CPT Codes: | 87389 x1 | ||
| Turnaround Time: | Performed Mon-Fri; results are reported within 3 days. | ||
| Special Instructions: | It is recommended that HIV testing be discussed with the patient prior to collection of the specimen. Written patient consent for HIV testing is not required. | ||
| Associated Links: | |||
Collection Instructions
| Specimen: | Blood |
| Optimal Volume: | 2 mL |
| Minimum\Peds Volume: | 1 mL |
| Container: | Red or gold (gel) Alternate Containers: Green (lithium heparin, no gel), Green (lithium heparin, gel), Purple (EDTA), Red (no gel) |
| Causes for Rejection: | Grossly hemolyzed, heat inactivated, microbial contamination |
Processing and Shipping
| Specimen Processing: | Within 2 hours of collection, centrifuge and aliquot 0.5 mL, 0.35 mL minimum. |
| Shipping Instructions: | Ship at refrigerated temperature. |
| Stability: | After separation from cells: Up to 7 days refrigerated. Freeze aliquot sample if stored for more than 7 days. |
| Test Performed at or Referral Lab | UM Specialty Core/Prot/Endo (Architect A) |
Interpretive
| Reference Range: |
The testing algorithm begins with the Abbott HIV-1/2 AG/AB screening immunoassay that simultaneously detects both antibody to human immunodeficiency virus (HIV) and HIV p24 antigen on the Abbott Architect instrument, performed at the East Bank campus. If the patient specimen is repeatedly reactive with the screening assay, it will be tested using the HIV Geenius (BioRad) HIV-1/2 immunoassay, which can differentiate between HIV-1 and HIV-2 antibodies. If the differentiation assay is also reactive, the presence of HIV-1 or HIV-2 antibodies is established. Specimens non-reactive with the HIV Geenius are reported as indeterminate and further testing with a qualitative or quantitative nucleic acid amplification test (NAT) should be performed to identify or rule out acute HIV-1 infection. If both HIV-1 and HIV-2 are positive, additional testing is required to rule out dual infection. The HIV Geenius CPT code is 86701 and 86702 and the Epic Code is LAB6328. The HIV Geenius will only be ordered by the Laboratory. Additional HIV testing is required to rule out dual infection.
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| Use: | This test is not recommended for patients younger than 18 months of age. If an HIV infection is suspected in a patient younger than 18 months of age, it is recommended to perform nucleic acid amplification testing. |
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