Universal PCR for Bacteria by 16S Ribosomal Next Generation Sequencing
| Sunquest Code: | NGS16S | ||
| Epic Code: | LAB6519 | Epic Name: | Bacterial DNA 16S Ribosomal Non Blood |
| Synonyms: | 16S rRNA | ||
| Methodology: | DNA extraction, nucleic acid purification, polymerase chain reaction (PCR), next generation sequencing | ||
| Turnaround Time: | Results are reported within 5-10 days | ||
| Special Instructions: | Infectious Diseases Diagnostic Laboratory Medical Director approval is required. | ||
Collection Instructions
| Specimen: | Fresh or paraffin embedded tissue; sterile body fluid (BAL or CSF) |
| Optimal Volume: | Tissue 1 cc (preferred); 1 mL fluid |
| Container: | Container, Tissue, sterile (Plastic leakproof container, sterile) |
| Causes for Rejection: | Sputum, swabs, tissue free-floating in formalin; bloody samples or samples containing citrate or heparin. |
Processing and Shipping
| Specimen Processing: | Do not process. Store fresh tissue or body fluid frozen; paraffin embedded tissue at room temperature. |
| Shipping Instructions: | Ship fresh tissue or sterile tissue frozen; paraffin embedded tissue at room temperature. |
| Test Performed at or Referral Lab | Lab Sendouts (University of Washington Medical Center) |
| Referral Lab number: | NGS16S |
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