Bacterial DNA 16S Ribosomal Non Blood with reflex to NGS 16S with reflex to Enterobacterales ID

Abbrev Code:BCTPCR   
Order Code:LAB6518Order Name:Bacterial DNA 16S Ribosomal Non Blood with reflex to NGS 16S with reflex to Enterobacterales ID
Synonyms:Bacterial DNA 16S Ribosomal replaces Explify Respiratory RNA Pathogen Detection; Bacterial Detection of Direct Specimen
Methodology:DNA extraction, nucleic acid purification, polymerase chain reaction (PCR); Next Generation Sequencing, if reflexed.
CPT Codes: 87801 x1, 87801 x1, 87801 x1
Turnaround Time:Specimens are sent to reference laboratory Mon-Fri; results are reported within 2-16 days depending on reflexes.
Special Instructions:Infectious Diseases Diagnostic Laboratory Medical Director approval is required for add-on test from an organism or specimen being retained in IDDL.

Collection Instructions

Specimen:Fresh or paraffin embedded tissue; sterile body fluid; swabs
Optimal Volume:Tissue 1 cc (preferred); 1 mL fluid
Container:Container, Tissue, sterile
Alternate Containers: Sterile tube, Plastic leakproof container, sterile, Eswab Copan, Purple (EDTA), Viral Transport Media
Collection Instructions:Tissue or fluid placed in a sterile container. Also acceptable: Formalin-fixed paraffin-embedded tissues (FFPE, PET); non-blood body fluids collected in Vacutainer tubes without preservative; EDTA tubes. E-swabs and UTM (Universal Transport medium) are acceptable for BCTPCR testing and ENBDNA testing, but not NGS testing.
Causes for Rejection:Tissues free-floating in formalin; samples containing citrate, or heparin; Blood/Serum specimens; swab/fluid collected in tube containing agar. SPS not acceptable for NGS testing.

Processing and Shipping

Specimen Processing:Do not process. Store frozen.
Shipping Instructions:Ship all specimens on dry ice.
Test Performed at or Referral Lab Lab Sendouts  (University of Washington Medical via IDDL Micro UMMC East)
Referral Lab number:BCTDNA, NGS16S, ENBDNA

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