Bordetella pertussis Antibodies IgA and IgG by ELISA with Reflex to Immunoblot


Abbrev Code:BPTAGE   
Order Code:LAB6759Order Name:B Pertussis IGA IGG ELISA RFLX to IMMBLT
Synonyms:B Pertussis
Methodology:Enzyme-linked immunosorbent assay/Immunoblot
CPT Codes: 86615 x2, 86615 x1
Turnaround Time:Specimens are sent to reference laboratory Sun-Sat; results are reported within 1-5 days.
Special Instructions:Parallel testing is preferred, and convalescent specimens must be received within 30 days from receipt of the acute specimens. Mark specimens plainly as "acute" or "convalescent".
Compliance:

This test uses a manufacturer labeled Analyte Specific Reagent (ASR) as the reagent providing the specificity of the assay. The test was developed and its performance characteristics determined by ARUP Laboratories. It has not been cleared or approved by the US Food and Drug Administration. This test was performed in a CLIA certified laboratory and is intended for clinical purposes.


Collection Instructions

Specimen:Blood
Optimal Volume:2 mL
Minimum\Peds Volume:0.8 mL
Container:Red or gold (gel)
Alternate Containers: Red (no gel)
Causes for Rejection:Contaminated, heat-inactivated, icteric, turbid or severely lipemic specimens


Processing and Shipping

Specimen Processing:Centrifuge and aliquot ASAP or within 2 hours of collection, 1 mL, 0.3 mL minimum. Store in refrigerator. Mark samples clearly as acute or convalescent.
Shipping Instructions:Ship at refrigerated temperature.
Stability:After separation from cells: 48 hours at room temperature; 2 weeks refrigerated; 1 year frozen.
Test Performed at or Referral Lab Lab Sendouts  (ARUP)
Referral Lab number:2001774


Interpretive

Reference Range:
Bordetella pertussis Aby, IgA by ELISA:
0.9 IV or less Negative: No significant level of detectable B. pertussis IgA Aby
1.0-1.1 IV Equivocal: Repeat testing in 10-14 days may be helpful.
1.2 IV or greater      Positive: IgA antibody to B. pertussis detected, which may indicate a current or past exposure/immunization to  B. pertussis.

Bordetella pertussis Aby, IgA by Immunoblot: Negative

Bordetella pertussis Aby IgG by ELISA:
0.94 IV or less Negative: No significant level of detectable  B. pertussis IgG antibody.
0.95-1.04 IV Equivocal: Repeat testing in 10-14 days may be helpful.
1.05 IV or greater       Positive: IgG antibody to B. pertussis detected, which may indicate a current or recent exposure/immunization to B. pertussis.

Bordetella pertussis Aby, IgG by Immunoblot: by report.
Use:If Bordetella pertussis Antibody, IgA by ELISA is 1.2 IV or greater, than Bordetella pertussis IgA Immunoblot testing will be added.
If Bordetella pertussis Antibody, IgG by ELISA is 1.05 IV or greater, then Bordetella pertussis IgG Immunoblot testing will be added.
Additional charges apply.


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