KIT (D816V) Mutation
| Abbrev Code: | NGSON | ||
| Order Code: | LAB6497 | Order Name: | Next Generation Sequencing |
| Methodology: | Next generation sequencing | ||
| CPT Codes: | 81272 x1 | ||
| Turnaround Time: | Performed Mon-Fri; results are reported within 10-14 days. | ||
| Special Instructions: | Refer to requisition form for available tests (see Associated Links). For non-EPIC sites: Print out requisition(s) and check gene(s) or panel requested. | ||
| Associated Links: | MDL Oncology Tumor Marker Request Form, NGSON Order Instructions |
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Collection Instructions
| Specimen: | Blood or bone marrow
Fixed tissue slides (preferred); fresh tissue |
| Optimal Volume: | 10 mL blood or 3 mL bone marrow
Fixed tissue; 5 unstained slides (10 microns thick), 1 H&E slide (10 microns thick) fixed tissue; fresh tissue: 5 mm x 5 mm x 5 mm. |
| Minimum\Peds Volume: | 3 mL blood or 1 mL bone marrow
Fixed tissue; 4 unstained slides (10 microns thick), 1 H&E slide (10 microns thick) fixed tissue; fresh tissue: 3 mm x 3 mm x 3 mm. |
| Container: | Yellow (ACD, Solution A) tube available from laboratory Alternate Containers: Purple (EDTA) |
| Collection Instructions: | Follow the NGSON Order Instructions prior to sample collection. See Associated Links. |
| Causes for Rejection: | Whole blood: Frozen or clotted specimen, incorrect anticoagulant, specimen more than 5 days old. Add on testing to hematology samples is not accepted. |
Processing and Shipping
| Specimen Processing: | Do not process. Store at room temperature. |
| Shipping Instructions: | Ship at room temperature. Do not refrigerate or freeze. |
| Test Performed at or Referral Lab | Molecular Diagnostics (UMMC East Bank) |
Interpretive
| Reference Range: | See interpretive report. |
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