Abbrev Code:FE   
Order Code:LAB94Order Name:Iron
Methodology:Roche: Colorimetric
Vista: Bichromatic endpoint
CPT Codes: 83540 x1, 83540 x1
Turnaround Time:Performed and reported 24 hours/day.
Associated Links:

Roche Go Live Schedule

Collection Instructions

Optimal Volume:2.5 mL
Minimum\Peds Volume:0.6 mL
Collection Instructions:Chemistry Tests Containers Chart

Collect sample before patient is given therapeutic iron or a blood transfusion. Blood transfusion can transiently (<24 h) increase serum iron concentration and transferrin saturation (but not total iron binding capacity), particularly if the blood has been stored for a longer period (Blood 2011 Dec 15; 118(25):6675-82; Arch Pathol Lab Med 1993 Jun; 117(6):(622-4).Therefore, if a patient has received blood, delay testing for 24 hours to avoid false elevation of iron or transferrin saturation. Calculation for Transferrin Saturation: (serum iron/TIBC) x 100.
Causes for Rejection:Moderate hemolysis
Contraindications:Falsely elevated iron levels will result if the patient has received therapeutic iron within the past two months. Patients treated with metal-binding drugs such as Deferoxamine may have depressed iron values as chelated iron may not properly react in the iron assay.

Processing and Shipping

Specimen Processing:Centrifuge within 4 hours and aliquot 1.25 mL, 0.2 mL minimum. Store in refrigerator.
Shipping Instructions:Ship at refrigerated temperature.
Stability:Roche: 3 weeks refrigerated; 2 years frozen.
Vista: 7 days refrigerated; 6 months frozen.  
Test Performed at or Referral Lab Chemistry  (CSC Maple Grove, Roche-CSC Mpls, Roche-Grand Itasca, Roche-Lakes, Roche-Northland, Roche-Range, Roche-Ridges, Roche-Southdale, Roche-St. John's, Roche-UMMC East, Roche-UMMC West)


Reference Range:
Site Age μg/dL
Woodwinds All 42-175
Vista 0-6 d 110-270
Vista 7-30 d 80-156
Vista 1-5 mo 25-115
Vista 6 mo-13 y 25-140
Vista 14 y & older 35-180
Roche Female 37-145
Roche Male 61-157

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