Measles Real-time Polymerase Chain Reaction (RT-PCR) Confirmation to MDH

Abbrev Code:MEAPCR   
Order Code:LAB6861Order Name:Measles Confirmation PCR
Methodology:Real-time polymerase chain reaction (PCR)
CPT Codes: x0
Special Instructions:MDH lab routinely tests for both measles and rubella when specimens are submitted for either disease.. All suspect cases of measles and specimens submitted for testing must be coordinated with an MDH epidemiologist at 651-201-5414 or toll-free at 1-877-676-5414.
Associated Links:

MDH Submission Form

Collection Instructions

Specimen:Order of preference: 1) throat swab; 2) nasal, nasopharyngeal swab or nasal wash; 3) urine specimen
Optimal Volume:See collection instructions
Minimum\Peds Volume:See collection instructions
Container:See Collection Instructions
Alternate Containers: Plastic leakproof container, sterile
Collection Instructions:
Throat swab: Vigorously swab tonsillar areas and posterior nasopharynx with a viral culturette. Use tongue blade to depress tongue to prevent contamination of swab with saliva. Place swab into viral transport medium (see Approved Swabs and Media).
Nasal or nasopharyngeal swab: Use sterile swabs to swab the nasal passage or the nasopharynx with either a viral culture swab or culturette. Place swab into viral transport media.
Nasal wash (two techniques): Use a syringe attached to a small plastic tube and 2 mL of viral transport medium. After placing viral transport medium in the nostril, aspirate as much of the material as possible and rinse the tube with the original (2 mL) of viral transport medium. - or - Use sterile swabs to swab the nasopharynx and throat with either a viral culture swab or culturette. Place both swabs in a tube containing 2-3 mL of transport medium. The virus is extremely cell-associated, so attempt to swab the throat and nasal passages to collect epithelial cells. After swabbing, place the swab back in the transport sheath.
Urine specimen: Collect 10-40 mL of urine in a sterile 50 mL centrifuge tube or a urine specimen container. First-morning voided specimens are ideal, but any urine collection is adequate. Have the patient void directly into the container, collection from the first part of the urine stream if possible.  

Processing and Shipping

Specimen Processing:


·          Do not process. Store in refrigerator.

·          Send specimen with a completed MDH submission form.

·          Package the specimen utilizing category B shipping instructions.

·          Label the package for delivery to MDH and ship directly to MDH.



Shipping Instructions:Ship overnight at refrigerated temperature. Enclose the MDH submission form with specimen.
Test Performed at or Referral Lab (Minnesota Department of Health)

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