HIV 1/2 Antigen/Antibody Pre-Transplant only


Abbrev Code:HIGBNC   
Order Code:LAB6466Order Name:HIV Antigen Antibody Combo Pretransplant
Synonyms:Human Immunodeficiency Virus Antigen/Antibody Combination
Methodology:Chemiluminescent immunoassay
CPT Codes: 87389 x1
Turnaround Time:Performed Mon-Fri; results are reported within 3 days.
Special Instructions:It is recommended that HIV testing be discussed with the patient prior to collection of the specimen. Written patient consent for HIV testing is not required.
Associated Links:

HIV Diagnostic Algorithm



Collection Instructions

Specimen:Blood
Optimal Volume:3 mL
Minimum\Peds Volume:2.2 mL
Container:Red or gold (gel)
Alternate Containers: Green (lithium heparin, no gel), Green (lithium heparin, gel), Purple (EDTA), Red (no gel)
Causes for Rejection:Grossly hemolyzed, heat-inactivated or microbial contamination.


Processing and Shipping

Specimen Processing:Centrifuge and aliquot 1.5 mL, 1 mL minimum.
Shipping Instructions:Ship at refrigerated temperature.
Stability:After separation from cells: Up to 7 days refrigerated.
Test Performed at or Referral Lab Specialty Core  (UMMC-East Bank)


Interpretive

Reference Range:
The testing algorithm begins with the Abbott HIV-1/2 AG/AB screening immunoassay that simultaneously detects both antibody to human immunodeficiency virus (HIV) and HIV p24 antigen on the Abbott Architect instrument, performed at the East Bank campus. If the patient specimen is repeatedly reactive with the screening assay, it will be tested using the HIV Geenius (BioRad) HIV-1/2 immunoassay, which can differentiate between HIV-1 and HIV-2 antibodies. If the differentiation assay is also reactive, the presence of HIV-1 or HIV-2 antibodies is established. Specimens non-reactive with the HIV Geenius are reported as indeterminate and further testing with a qualitative or quantitative nucleic acid amplification test (NAT) should be performed to identify or rule out acute HIV-1 infection. If both HIV-1 and HIV-2 are positive, additional testing is required to rule out dual infection.      


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