PRA, Donor Specific Antibody
| Abbrev Code: | PRADSA | ||
| Order Code: | LAB6445 | Order Name: | PRA, Donor Specific Aby |
| Order Instructions: | If this test is being added on, comment ADD-ON in the Comment field when placing a normal order in Epic. Immunology will receive the printout directly. All other lab areas should discard the Immunology ADD-ON printout. | ||
| Synonyms: | HLA Class I/II Antibody; PRA (Panel Reactive Antibody); WBC Antibody; White Cell Antibody; Antileukocyte Antibody; IMM3 | ||
| Methodology: | Luminex solid phase assay | ||
| CPT Codes: | 86832 x1, 86832 x1, 86833 x1, 86833 x1 | ||
| Turnaround Time: | Results are reported within 7 days. | ||
| Special Instructions: | If testing is requested on a patient that has received a transplant at an outside institution, the following information must be provided: Recipient HLA type, Donor HLA type. | ||
Collection Instructions
| Specimen: | Blood |
| Optimal Volume: | 14 mL |
| Minimum\Peds Volume: | 3 mL |
| Container: | Red (no gel) Alternate Containers: Red (gel) |
| Collection Instructions: | Collect specimen prior to dialysis. Do not collect specimen if patient has received IVIG, OKT3 or Campath recently; these drugs may interfere with testing. |
| Causes for Rejection: | Gross hemolysis; immunosuppression drug interference. |
| Contraindications: | Serum will not clot if collected during or immediately after dialysis. |
Processing and Shipping
| Specimen Processing: | Do not process (preferred). If processed, remove serum. Store at room temperature. |
| Shipping Instructions: | Ship at room temperature. Protect from temperature extremes. |
| Stability: | Store at room temperature (preferred), refrigerated or frozen. |
| Test Performed at or Referral Lab | Immunology (UMMC East Bank) |
Interpretive
| Use: | Detection of HLA Class I and/or Class II antibodies in patient serum. High levels of HLA antibody may result in difficulty in finding a compatible organ or marrow donor. |
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