Leukemia Lymphoma Evaluation, Cerebrospinal Fluid (CSF)

Sunquest Code:FLOLL  
Epic Code:LAB501Epic Name:Immunophenotyping/Flow Cytometry CSF (Epic)
Sunquest Code (non-Epic sites):FLOWCSF  
Synonyms:Immunophenotpying CSF; Flow Cytometry CSF
Methodology:Multi-color immunofluorescence and flow cytometry
Test Includes:Combination of monoclonal antibodies chosen by pathologist for detection of malignant hematologic neoplasms, most commonly lymphoma or acute leukemia. Specific combination of monoclonal antibodies may include B lymphocyte markers including kappa and lambda/ HLA-DR, monocytic and/or myeloid markers, T lymphocyte markers, immature cell markers, or plasma cell markers. Results are interpreted by pathologist.
Turnaround Time:Performed Mon-Fri, 8 am to 5 pm. Results are reported within 1-2 days.
Special Instructions:Provide diagnosis, age, collection date and time, WBC and leukocyte differential. Deliver to the IFC laboratory within 4 hours of collection. Should not be performed on the first tube of collection.

This test was developed and its performance characteristics determined by University of Minnesota Medical Center, Fairview Clinical Laboratories. It has not been cleared or approved by the US Food and Drug Administration. FDA does not require this test to go through premarket FDA review. This test is used for clinical purposes and should not be regarded as investigational or for research. This laboratory is certified under the Clinical Laboratory Improvement Amendments (CLIA) as qualified to perform high complexity clinical testing.

Collection Instructions

Optimal Volume:5 mL
Minimum\Peds Volume:3 mL (adult); 1 mL (pediatric)
Container:Sterile tube
[Alternate Containers: Plastic leakproof container, sterile]
Collection Instructions:
Sample must be received by the Immunophenotyping and Flow Cytometry laboratory within 4 hours of collection. Perform testing on the 3rd or 4th tube collected.  
Causes for Rejection:Samples received frozen, spun or too old to obtain accurate results (per pathologist decision).

Processing and Shipping

Specimen Processing:Do not process. Contact IFC laboratory with any questions prior to sending specimen at 612-273-5248. Testing should not be performed on the first tube of collection. Perform testing on the 3rd or 4th tube collected.
Shipping Instructions:Ship ASAP after collection. Must arrive at testing laboratory within 4 hours of collection.
Test Performed at or Referral Lab Immunophenotyping and Flow Cytometry  (UMMC-East Bank)


Reference Range:By report.
Use:Diagnosis and clinical management of acute and chronic lymphoid and non-lymphoid leukemias. Monitor response to treatment and recurrent disease relapses. Define the type of purging procedure to be done prior to bone marrow reinfusion in autologous BMT. Identifies various types and subtypes of malignant lymphomas, such as B-cell, T-cell and true histiocytic. Differentiates lymphomas from other cancers and non-malignant processes.

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