Urine Culture, Catheterized

Sunquest Code:URC  
Epic Code:LAB239Epic Name:Urine Culture
Methodology:Quantitative culture
Test Includes:Culture of aerobic flora from 100 to >100,000 organisms/mL and susceptibility testing. Gram stain if requested.
Turnaround Time:Performed daily; results are reported within 2 days.

Collection Instructions

Specimen:Urine, catheterized collection
Optimal Volume:5-10 mL
Minimum\Peds Volume:0.2 mL (4.0 mL min if using vacutainer C&S)
Container:Urine culture tubes
Collection Instructions:

Refrigerate specimen if not delivered to laboratory within 30 minutes or collect in vacutainer urine C&S transport kit.

·          CATHETER COLLECTION: Clean catheter with alcohol sponge, puncture with sterile needle, and collect urine in sterile syringe. (Note: This technique is unsatisfactory for certain silicone catheters which may then leak).

·          SUPRAPUBIC TAP: Expose area above pubis. Scrub area with povidone iodine. Cover area with povidone iodine solution; allow to dry. Using sterile syringe and needle, aspirate urine from bladder, inject into sterile container. (If anaerobic culture is desired, expel air bubble and inject sample into anaerobic vial).


Contraindications:Bladder or urethral catheterization for culture may introduce infection, and has been generally replaced by clean catch technique when feasible. Do not collect urine from drainage bag of indwelling catheter, because bacterial growth can occur in bag. Catheter tips are contaminated by urethra as they are withdrawn and should not be cultured.

Processing and Shipping

Specimen Processing:Store in refrigerator. Specimen must arrive within 24 hours. Vacutainer: store at room temperature. Specimen must arrive within 48 hours.
Shipping Instructions:Ship at refrigerated temperature. Must arrive within 24 hours. Vacutainer: ship at room temperature. Must arrive within 48 hours.
Test Performed at or Referral Lab Infectious Diseases Diagnostic Laboratory  (Fairview Range - FRMC, UMMC-East Bank, Grand Itasca GICH)


Reference Range:No growth.
Use:Isolate and identify potentially pathogenic organisms causing urinary tract infection.

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