Colorectal Cancer Panel
| Abbrev Code: | NGSON | ||
| Order Code: | LAB6497 | Order Name: | Next Generation Sequencing Oncology |
| Synonyms: | KRAS, BRAF, HRAS, NRAS, PIK3CA | ||
| Methodology: | Next generation sequencing and FISH testing | ||
| CPT Codes: | 81445 x1, G0452 x1 | ||
| Test Includes: | Next generation sequencing of KRAS, BRAF, HRAS, NRAS, PIK3CA. | ||
| Turnaround Time: | Performed Mon-Fri; results are reported within 10-14 days. | ||
| Special Instructions: | Refer to requisition form for available tests. (See Associated Links.) Non-Epic sites print out requisition(s) and check gene(s) or panel requested. Order testing in EPIC as colorectal panel. Fixed samples: include block number for testing. | ||
| Associated Links: | MDL Oncology Tumor Marker Request Form, NGSON Order Instructions |
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Collection Instructions
| Specimen: | Fixed tissue (preferred); fresh tissue |
| Optimal Volume: | Fixed tissue: 5 unstained slides (10 microns thick) and 1 H&E slide (10 microns thick) fixed tissue. Fresh tissue: 5 mm x 5 mm x 5 mm. |
| Minimum\Peds Volume: | Fixed tissue: 4 unstained slides (10 microns thick) and 1 H&E slide (10 microns thick) fixed tissue. Fresh tissue: 3 mm x 3 mm x 3 mm. |
| Container: | Plastic leakproof container, sterile |
| Collection Instructions: | Follow the NGSON Order Instructions prior to sample collection. See Associated Links. |
| Causes for Rejection: | Decalcified tissue. |
Processing and Shipping
| Specimen Processing: | Do not process. Store at room temperature. |
| Shipping Instructions: | Ship at room temperature. Do not refrigerate or freeze. |
| Stability: | Do not refrigerate or freeze. |
| Test Performed at or Referral Lab | Molecular Diagnostics (UMMC-East Bank) |
Interpretive
| Reference Range: | See interpretive report. |
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