Acute Myeloid Leukemia Panel

Abbrev Code:NGSON   
Order Code:LAB6497Order Name:Next Generation Sequencing Oncology
Synonyms:Complete AML; Complete AML Neoplasm Panel; Short AML Panel; Short AML Neoplasm Panel; FLT3, NPM1, CEBPA, AML
Methodology:Next generation sequencing
CPT Codes: 81450 x1, G0452 x1, 81245 x1, 81310 x1, 81218 x1
Test Includes:Short AML panel includes next generation sequencing of FLT3, NPM1 and CEBPA genes. The complete panel includes next generation sequencing of FLT3, IDH1, IDH2, KIT, KRAS, NRAS, NPM1, PDGFRA, TP53, WT1 and CEBPA genes.
Turnaround Time:Performed Mon-Fri; results are reported within 10-14 days.
Special Instructions:Refer to requisition form for available tests. (See Associated Links). For non-EPIC sites: Print out requisition(s) and check gene(s) or panel requested. Order testing in EPIC as Complete or Short AML Panel.
Associated Links:

NGSON Order Instructions, Gene Specific CPT Codes for Genetic Sequencing

Collection Instructions

Specimen:Blood, bone marrow
Optimal Volume:10 mL blood; 3 mL bone marrow
Minimum\Peds Volume:3 mL blood; 1 mL bone marrow
Container:Yellow (ACD, Solution A) tube available from laboratory
Alternate Containers: Purple (EDTA)
Collection Instructions:Follow the NGSON Order Instructions prior to sample collection. See Associated Links.
Causes for Rejection:Whole blood: frozen or clotted specimen, incorrect anticoagulant, specimen more than 5 days old. CEBPA will be cancelled if tumor percentage is less than 30%. All testing will be cancelled if engraftment testing is 100% donor engrafted in consultation with clinician. DNA extracted at non-CLIA certified (or equivalent) labs.
Shared blood or bone marrow samples will not be accepted unless sent to Molecular first. Contamination will occur on automated hematology analyzers.
The diagnostic sample for hematology panels (not including MPN) requires at least 20% blasts to perform testing. Follow-up samples for the hematology panels will be tested for all the gene(s) positive in the diagnostic sample and FLT3. Any genes testing negative in the diagnostic sample will be cancelled.

Processing and Shipping

Specimen Processing:Do not process. Store at room temperature.
Shipping Instructions:Ship at room temperature. Do not refrigerate or freeze.
Stability:Whole blood samples are stable for 5 days at room temperature. Do not refrigerate or freeze.
Test Performed at or Referral Lab UM Molecular Diagnostics (M)  (UMMC East Bank)


Reference Range:See interpretive report.

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