Semen Analysis Using Assisted Reproductive Technology (ART) Processing - LG6337

Epic Code:LAB90561Epic Name:ART Processing
Methodology:Macroscopic and microscopic analysis
Test Includes:Liquefaction, viscosity, pH, volume, concentration, total number, motility, vitality, round cell concentration, and morphological interpretation of stained smear. Complex analysis using ART processing to assess pre- and post-processing morphology and sperm motility after 18-22 hours of incubation.
Turnaround Time:Performed Mon-Fri and by appointment only; results are reported within 5 business days.
Special Instructions:For further information, contact Diagnostic Andrology Laboratory at 612-672-4080; for patient scheduling contact 612-672-4072.

Collection Instructions

Optimal Volume:Submit entire collection.
Container:Container, Semen
Collection Instructions:Physician instructs patient to abstain from ejaculation for 2-7 days before collection. Patient should collect specimen on-site. In limited circumstances the patient may collect specimen off-site. The laboratory provides instructions for off-site collection.

Processing and Shipping

Test Performed at or Referral Lab (Diagnostic Andrology Laboratory)


Reference Range:


Grey-opalescent to opaque, initially viscous


15-60 minutes


Greater than or equal to 7.2


1.5 mL or greater


15 million/mL or greater

Total Sperm number

39 million or greater

Total motility (non-progressive and progressive)

Greater than or equal to 40%

Progressive motility

Greater than or equal to 32%

Leukocyte concentration

Less than 2 million/mL


58% or greater

WHO laboratory manual for the examination and processing of human semen-5th edition, 2010.


Use:Infertility studies, post vasectomy studies: diagnosis of azoospermia, oligozoospermia, asthenozoospermia.

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