Retrograde Semen Analysis

Order Code:LAB90556Order Name:Retrograde Semen Analysis
Methodology:Macroscopic and microscopic analysis
CPT Codes: 89331 x1
Test Includes:Liquefaction, viscosity, pH, volume, concentration, total number, motility, vitality, round cell concentration, and morphological interpretation of stained smear. Analysis of urine for present of sperm.
Turnaround Time:Performed Mon-Fri and by appointment only; results are reported within 5 business days.
Special Instructions:For further information, contact Diagnostic Andrology Laboratory at 612-672-4080; for patient scheduling contact 612-672-4072.

Collection Instructions

Specimen:Semen and urine
Optimal Volume:Submit entire semen collection in one container and entire urine collection in second container.
Container:Container, Semen, Plastic leakproof container, sterile
Collection Instructions:Refer to collection procedure for sperm evaluation, semen analysis and instruction from lab.
Causes for Rejection:Specimen greater than 1 hour old. Unapproved container. Improper collection method.

Processing and Shipping

Test Performed at or Referral Lab (Diagnostic Andrology Laboratory)


Reference Range:


Grey-opalescent to opaque, initially viscous


15-60 minutes


Greater than or equal to 7.2


1.5 mL or greater


15 million/mL or greater

Total Sperm number

39 million or greater

Total motility (non-progressive and progressive)

Greater than or equal to 40%

Progressive motility

Greater than or equal to 32%

Leukocyte concentration

Less than 2 million/mL


58% or greater

WHO laboratory manual for the examination and processing of human semen-5th edition, 2010.



Use:Infertility studies, post vasectomy studies: diagnosis of azoospermia, oligozoospermia, asthenozoospermia.

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