Epidermal Nerve Fiber

Abbrev Code:ENF   
Order Code:LAB6605Order Name:Epidermal Nerve Fiber Density
Order Instructions:Only one Epidermal Nerve Fiber order is required for multiple samples. Order one ENF and transport all biopsies together.
Synonyms:ENF; Skin Biopsy for Nerve Density; Confocal Microscopy
Methodology:Immunofluorescent localization of nerve/tissue antigens in skin sections, confocal microscopy, tracing and quantification of epidermal nerve fibers, report of nerve fiber density, general innervation morphology
CPT Codes: 88314 x1, 88305 x1, 88346 x1, 88348 x1, 88356 x1, 88350 x5
Turnaround Time:Specimens are sent to reference laboratory Mon-Thur; results are reported within 15 days
Special Instructions:STAT processing required. Sample must be in Zamboni fixative within 4 hours and arrive in performing lab within 24 hours. Place tissues directly into Zamboni fixative (available from Kennedy Lab 612-625-1431) immediately upon excision and send to UMMC-East Core Lab for delivery to the Kennedy Laboratory, J-119 Diehl Hall.

Collection Instructions

Specimen:Tissue-Skin Biopsy
Optimal Volume:3 mm
Container:Zamboni Fixative Vial
Collection Instructions:Place tissues directly into Zamboni fixative-containing vial immediately upon excision. Place vial with specimen in stryrofoam container with an ice pack.
Causes for Rejection:Damaged tissue, frozen tissue, tissue not in fixative.

Processing and Shipping

Specimen Processing:STAT processing required. See Special Instructions.
UMMC East Bank Core: Check in sample and call Kennedy for pick up ASAP. Log sample on the Pass-Thru Log and store refrigerated in the Pass-Thru bucket. Keep refrigerated; do not freeze.
Shipping Instructions:Ship at refrigerated temperatures. Do not freeze.
Test Performed at or Referral Lab Lab Sendouts  (Kennedy Lab, J-119 Diehl Hall)

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