Lymphocyte Proliferation to Anti-CD3/Anti-CD28 and Anti-CD3/Interleukin-2 (IL-2) by Flow Cytometry


Sunquest Code:LPA3FC  
Epic Code:LAB6786Epic Name:Lymphocyte Proliferation aCD3
Methodology:Flow cytometry
Turnaround Time:Specimens are sent to reference laboratory Mon-Fri; results are reported within 5-8 days.
Special Instructions:Specimen must be received at the testing laboratory by 4 pm on Friday. For best result, sample should arrive within 24 hours of collection. Date and time of draw and ordering physician name and phone number are required.
Compliance:

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.


Collection Instructions

Specimen:Blood
Optimal Volume:20 mL
Container:Green (sodium heparin, no gel)
Collection Instructions:

Send specimen in original container. Specimen minimum volumes are as follows:

Less than 3 mos.

1 mL minimum

3-5 y

3 mL; 1 mL minimum

6-18 y

5 mL; 1 mL minimum

18 y and older

20 mL; 1 mL minimum

 

For serial monitoring, the specimen should be drawn at the same time of day.

 

Causes for Rejection:Grossly hemolyzed specimens. Lithium heparin specimens. Refrigerated or frozen samples. Samples received at testing laboratory after 4 pm on Friday.


Processing and Shipping

Specimen Processing:Do not process.
Shipping Instructions:Ship in original container at room temperature to arrive at testing laboratory no later than 4 pm on Friday.
Stability:Stability: 48 hours at room temperature.
Test Performed at or Referral Lab Lab Sendouts  (Mayo Medical Laboratories)
Referral Lab number:LPA3P


Interpretive

Reference Range:

Viability of lymphhocytes at day 0

≥ 75.0%

Maximum proliferation of anti-CD3 as % CD45

≥ 19.4

Maximum proliferation of anti-CD3 as % CD3

≥ 20.3%

Maximum proliferation of anti-CD3 + anti-CD28 as % CD45

≥ 37.5%

Maximum proliferation of anti-CD3 + anti-CD28 as % CD3

≥ 44.6%

Maximum proliferation of anti-CD3 + IL-2 as % CD45

≥ 41.7%

Maximum proliferation of anti-CD3 + IL-2 as % CD3

≥ 46.2%

 

 



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