Lymphocyte Proliferation to Anti-CD3/Anti-CD28 and Anti-CD3/Interleukin-2 (IL-2) by Flow Cytometry

Abbrev Code:LPA3FC   
Order Code:LAB6786Order Name:Lymphocyte Proliferation aCD3
Methodology:Flow cytometry
CPT Codes: 86353 x2, 86353 x1
Turnaround Time:Specimens are sent to reference laboratory Mon-Fri; results are reported within 5-8 days.
Special Instructions:Specimen must be received at the testing laboratory by 4 pm on Friday. For best result, sample should arrive within 24 hours of collection. Date and time of draw and ordering physician name and phone number are required.

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.

Collection Instructions

Optimal Volume:20 mL
Container:Green (sodium heparin, no gel)
Collection Instructions:

Send specimen in original container. Specimen minimum volumes are as follows:

Less than 3 mos.

1 mL minimum

3-24 months

3 mL; 1 mL minimum

25 months-18 y

5 mL; 1 mL minimum

18 y and older

20 mL; 1 mL minimum


For serial monitoring, the specimen should be drawn at the same time of day.


Causes for Rejection:Grossly hemolyzed specimens. Lithium heparin specimens. Refrigerated or frozen samples. Samples received at testing laboratory after 4 pm on Friday.

Processing and Shipping

Specimen Processing:Do not process.
Shipping Instructions:Ship in original container at room temperature to arrive at testing location weekdays and no later than 4 pm on Friday.
Stability:48 hours at room temperature.
Test Performed at or Referral Lab Lab Sendouts  (Mayo Medical Laboratories)
Referral Lab number:LPA3P


Reference Range:

Viability of lymphhocytes at day 0

≥ 75.0%

Maximum proliferation of anti-CD3 as % CD45

≥ 19.4

Maximum proliferation of anti-CD3 as % CD3

≥ 20.3%

Maximum proliferation of anti-CD3 + anti-CD28 as % CD45

≥ 37.5%

Maximum proliferation of anti-CD3 + anti-CD28 as % CD3

≥ 44.6%

Maximum proliferation of anti-CD3 + IL-2 as % CD45

≥ 41.7%

Maximum proliferation of anti-CD3 + IL-2 as % CD3

≥ 46.2%



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